Therapeutic plasma exchange in multiple sclerosis patients with abolished interferon-beta bioavailability
| Autor: | Gintaras Kaubrys, Loreta Bagdonaite, Mindaugas Stoškus, Laimonas Griskevicius, Natasa Giedraitiene, Vilma Valceckiene, Rasa Kizlaitiene |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Myxovirus Resistance Proteins medicine.medical_specialty Multiple Sclerosis medicine.medical_treatment Biological Availability Pilot Projects In vivo Clinical Research Internal medicine Gene expression medicine Humans Whole blood biology Plasma Exchange Reverse Transcriptase Polymerase Chain Reaction Multiple sclerosis Biological activity General Medicine Interferon-beta Plasmapheresis Middle Aged medicine.disease Antibodies Neutralizing Bioavailability Neutralizing Antibodies Endocrinology biology.protein Female Antibody Biomarkers |
| Zdroj: | Medical science monitor, Warsaw : Medical Science International, 2015, vol. 21, p. 1512-1519 Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | Background: Neutralizing antibodies (NAb) to interferon-beta (IFN-b) are associated with reduced bioactivity and efficacy of IFN-b in multiple sclerosis (MS). The myxovirus resistance protein A (MxA) gene expression is one of the most appropriate markers of biological activity of exogenous IFN-b. We hypothesized that therapeutic plasma exchange (TPE) can restore the ability of IFN-b to induce the MxA mRNA expression and that maintenance plasmapheresis can sustain the bioavailability of IFN-b. Material/Methods: Eligible patients underwent 4 primary separate plasma exchange sessions. After the induction TPE sessions, they were transferred to maintenance plasmapheresis. Bioactivity of IFN-b was expressed as in vivo MxA mRNA induction in whole blood using RT-qPCR. Results: Six patients with low IFN-b bioavailability detected by the MxA mRNA response were included. Four patients became biological responders after induction plasmapheresis. In 2 patients an increase of MxA mRNA expression was found, but the values persisted below the cut-off and the patients remained as “poor biological re sponders”. The effect of maintenance plasmapheresis was transient: MxA mRNA expression values reverted to the baseline levels after 1–2 months. Conclusions: Therapeutic plasma exchange is able to restore the bioavailability of IFN-b in the majority of studied patients, but the effect of TPE on the IFN-b bioavailability was transient. |
| Databáze: | OpenAIRE |
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