Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system
| Autor: | Peta L. Clode, Tanya Armstrong, R.C.A. Thompson, H. Borowski |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Zoology
Ileum Microbiology Cell Line Host-Parasite Interactions Apicomplexa Microscopy Electron Transmission parasitic diseases medicine Parasite hosting Animals Humans Cecum Phylogeny Cryptosporidium parvum Life Cycle Stages biology Epithelial Cells biology.organism_classification In vitro Infectious Diseases medicine.anatomical_structure Cell culture Ultrastructure Microscopy Electron Scanning Protozoa Animal Science and Zoology Parasitology |
| Zdroj: | Parasitology. 137(1) |
| ISSN: | 1469-8161 |
| Popis: | SUMMARYCryptosporidium parvumis a zoonotic protozoan parasite that mainly affects the ileum of humans and livestock, with the potential to cause severe enteric disease. We describe the complete life cycle ofC. parvumin anin vitrosystem. Infected cultures of the human ileocecal epithelial cell line (HCT-8) were observed over time using electron microscopy. Additional data are presented on the morphology, development and behavioural characteristics of the different life-cycle stages as well as determining their time of occurrence after inoculation. Numerous stages ofC. parvumand their behaviour have been visualized and morphologically characterized for the first time using scanning electron microscopy. Further, parasite-host interactions and the effect ofC. parvumon host cells were also visualized. An improved understanding of the parasite's biology, proliferation and interactions with host cells will aid in the development of treatments for the disease. |
| Databáze: | OpenAIRE |
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