Cloning, expression and functional identification of secoisolariciresinol dehydrogenase gene from Dysosma versipellis callus
| Autor: | Yun Shen, Kebo Xie, Jian-Hua Zou, Ridao Chen, Jungui Dai |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Cloning
Candidate gene DNA Complementary macromolecular substances Biology Berberidaceae Enzyme assay Podophyllotoxin Complementary and alternative medicine Biochemistry NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases Complementary DNA Callus medicine biology.protein Pharmacology (medical) Cloning Molecular General Pharmacology Toxicology and Pharmaceutics Secoisolariciresinol dehydrogenase Gene Plant Proteins medicine.drug |
| Zdroj: | China Journal of Chinese Materia Medica. |
| ISSN: | 1001-5302 |
| DOI: | 10.4268/cjcmm20162414 |
| Popis: | Secoisolariciresinol dehydrogenase (SDH) is a key enzyme involved in the biosynthetic pathway of podophyllotoxin.In this study, two SDH candidate genes,SO282 and SO1223, were cloned from callus of Dysosma versipellis by homology-based PCR and rapid amplification of cDNA end (RACE).The SDH candidate genes were expressed in Escherichia coli and the subsequent enzyme assay in vitro showed that recombinant SO282 had the SDH activity. These results pave the way to the follow-up investigation of the biosynthetic of podophyllotoxin. |
| Databáze: | OpenAIRE |
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