Effects of sevoflurane anaesthesia on radioligand binding to monoamine oxidase-B in vivo
| Autor: | Peter Johnström, Lars Farde, Christer Halldin, Katarina Varnäs, Ryosuke Arakawa, Lars Eriksson, Sjoerd J. Finnema |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
positron emission tomography
monoamine oxidase-B Monoamine oxidase sevoflurane brain imaging Sevoflurane Xylazine 03 medical and health sciences Radioligand Assay 0302 clinical medicine Neuroscience and Neuroanaesthesia 030202 anesthesiology medicine Radioligand Animals Ketamine Monoamine Oxidase business.industry Metabotropic glutamate receptor 5 Brain Macaca fascicularis Anesthesiology and Pain Medicine Isoflurane Anesthesia Positron-Emission Tomography volatile anaesthetics Anesthetics Inhalation Models Animal Monoamine oxidase B business medicine.drug |
| Zdroj: | BJA: British Journal of Anaesthesia |
| ISSN: | 1471-6771 0007-0912 |
| Popis: | Background The molecular actions underlying the clinical effects of inhaled anaesthetics such as sevoflurane and isoflurane are not fully understood. Unexpected observations in positron emission tomography (PET) studies with [11C]AZD9272, a metabotropic glutamate receptor 5 (mGluR5) radioligand with possible affinity for monoamine oxidase-B (MAO-B), suggest that its binding is sensitive to anaesthesia with sevoflurane. The objective of the present study was to assess the effects of sevoflurane anaesthesia on the binding of [11C]AZD9272 and of [11C]L-deprenyl-D2, a radioligand selective for MAO-B in non-human primates (NHPs). Methods Altogether, 12 PET measurements were conducted with a high-resolution research tomograph using the ligands [11C]AZD9272 or [11C]L-deprenyl-D2 in six cynomolgus monkeys anaesthetised with sevoflurane or ketamine/xylazine. Results The specific binding of [11C]AZD9272 and [11C]L-deprenyl-D2 was markedly reduced during anaesthesia with sevoflurane compared with ketamine/xylazine. The reduction was 80–90% (n=3) for [11C]AZD9272 and 77–80% (n=3) for [11C]L-deprenyl-D2. Conclusions Sevoflurane anaesthesia inhibited radioligand binding to MAO-B in the primate brain. The observation of lower MAO-B binding at clinically relevant concentrations of sevoflurane warrants further exploration of the potential role of MAO-B related mechanisms in regulation of systemic blood pressure during anaesthesia. |
| Databáze: | OpenAIRE |
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