Motility of L5222 leukemia cells within the mesentery

Autor: Gisela Haemmerli, Peter Sträuli
Rok vydání: 1978
Předmět:
Zdroj: Virchows Archiv B Cell Pathology. 29:167-177
ISSN: 0340-6075
DOI: 10.1007/bf02899350
Popis: Microcinematography, together with histology at the semithin and ultrathin section levels, was utilized for the demonstration of the motile behavior of L5222 rat leukemia cells within a living tissue, the mesentery. On days 1–6 after intraperitoneal implantation of L5222 cells, a period corresponding to the life span of leukemic animals, the pieces of mesentery infiltrated in vivo by leukemia cells were transferred into Rose chambers and filmed in vitro. The movements of the leukemia cells within the mesentery were qualitatively identical to those performed on glass, on spot motility and locomotion, both being in the characteristic polarized configuration recorded in earlier studies. The type of locomotion within the mesentery, however, was impressively influenced by the fibrillar structure of the organ. While thin elastic fibers were crossed by the leukemia cells by means of shape adaptation, thick collagen bundles represented, in most cases, true obstacles. Frequent changes of direction, short tracks and overall low speed of movement resulted. The structure of the mesentery was changed in time, after implantation, by massive thickening of the collagen bundles. These changes together with increasing numbers of leukemia cells, macrophages, mast cells, granulocytes and lymphocytes reduced the motility of L5222 cells.
Databáze: OpenAIRE