Evaluation of white blood cell concentration, plasma fibrinogen concentration, and an agar gel immunodiffusion test for early identification of foals with Rhodococcus equi pneumonia
| Autor: | James L. Bowman, Steeve Giguère, Jorge A. Hernandez, Gaskin Jm, Corey Miller |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Immunodiffusion
animal diseases Fibrinogen Sensitivity and Specificity Microbiology Leukocyte Count Predictive Value of Tests Reference Values Rhodococcus equi biology.animal White blood cell parasitic diseases medicine Leukocytes Pneumonia Bacterial Animals Horses Prospective Studies General Veterinary biology Receiver operating characteristic biology.organism_classification Ouchterlony double immunodiffusion Antibodies Bacterial medicine.anatomical_structure Foal Animals Newborn Predictive value of tests biology.protein Horse Diseases Antibody Actinomycetales Infections medicine.drug |
| Zdroj: | Journal of the American Veterinary Medical Association. 222(6) |
| ISSN: | 0003-1488 |
| Popis: | Objective—To evaluate WBC concentration, plasma fibrinogen concentration, and an agar gel immunodiffusion (AGID) test for early identification of Rhodococcus equi-infected foals. Design—Prospective study. Animals—162 foals from a farm with enzootic R equi infection. Procedure—Blood samples were obtained from each foal at 4-week intervals for measurement of WBC and plasma fibrinogen concentrations and at 2-week intervals for detection of anti-R equi antibody by an AGID assay. Diagnostic performance of WBC and fibrinogen concentrations was assessed by use of receiver operating characteristic curve analysis. For each assay, sensitivity, specificity, and predictive values were calculated at various cutoff points; bacteriologic culture of R equi from a tracheobronchial aspirate was used as the reference standard test. Results—Diagnostic performance of WBC concentration was significantly higher than that of fibrinogen concentration. Sensitivity and specificity of measurement of WBC concentration at a cutoff of 13,000 cells/µL were 95.2 and 61.2%, respectively; at a cutoff of 15,000 cells/µL, sensitivity was 78.6% and specificity was 90.8%. When a positive test result was used as the cutoff, sensitivity of the AGID assay was 62.5% and specificity was 53.8%. Conclusion and Clinical Relevance—Monitoring WBC concentration is a useful approach for early detection of infected foals on farms with a high prevalence of R equi pneumonia. In contrast, serologic surveillance by use of an AGID assay is of little benefit for that purpose. (J Am Vet Med Assoc 2003;222:775–781) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|