Cell surface antigens reacting with antiretrovirus sera on normal mouse spleen cells: a flow cytometric study
| Autor: | Gábor Szabó, Ferenc D‐tóth, Antal Réthy |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Biophysics
Mitosis Spleen Mice Inbred Strains Biology Cross Reactions Immunofluorescence Antibodies Viral Rauscher Virus Pathology and Forensic Medicine Flow cytometry Mice Endocrinology Antigen medicine Animals Antigens Viral Interphase Antiserum CD40 Leukemia Experimental medicine.diagnostic_test Cell Biology Hematology Cell cycle Flow Cytometry Molecular biology medicine.anatomical_structure Antigens Surface biology.protein Antibody |
| Zdroj: | Cytometry. 4(1) |
| ISSN: | 0196-4763 |
| Popis: | The development of erythroleukemia in Balb/c mice injected with Rauscher leukemia virus has been followed by indirect immunofluorescence technique and flow cytometry, using antiserum against disrupted of virions. The progression of the disease was accompanied by a great increase in the number of large, immunofluorescence positive cells. A subpopulation of normal spleen cells was also highly positive. The expression of the antigens in normal cells was examined in relation to the cell cycle. The majority of the S-G2/M phase cells were found in the antigen positive compartment of larger cells. A two-color analysis of immunofluorescence and DNA content revealed that the distribution of antigen expression in G1 phase was broad, gradually decreasing from a low-intensity mode. The cell with double DNA content distributed evenly around a modus of five-fold higher intensity. In experiments with stimulated bone-marrow cells, superiority of S-phase cells in anti-Rauscher serum binding was found. Cell-surface gp70 antigen is suggested to be involved in this cell-cycle dependent binding of antibodies by normal cells. |
| Databáze: | OpenAIRE |
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