Association of venous thromboembolism and myocardial infarction with Factor V Leiden and Factor II gene mutations among Libyan patients
| Autor: | Ahmed Zaid, Ibtesam Khalil, Abushawashi Ali, Murad Ghrew, Abdulghani Msalati, Khaled Sedaa, Abdulla Bashein |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty Genotype Deep vein Myocardial Infarction Single-nucleotide polymorphism Gene mutation Thrombophilia Polymorphism Single Nucleotide Gastroenterology 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Factor V Leiden Humans Genetic Predisposition to Disease cardiovascular diseases 030212 general & internal medicine thrombophilia Venous Thrombosis Gene polymorphisms prothrombin G20210A business.industry Factor V Venous Thromboembolism General Medicine Middle Aged medicine.disease Thrombosis SNP genotyping medicine.anatomical_structure Heart Disease Risk Factors Medicine Prothrombin G20210A Female Prothrombin Research-Article Original Article business 030217 neurology & neurosurgery |
| Zdroj: | Libyan Journal of Medicine; Vol. 16 No. 1 (2021) The Libyan Journal of Medicine article-version (VoR) Version of Record Libyan Journal of Medicine, Vol 16, Iss 1 (2021) |
| ISSN: | 1819-6357 1993-2820 |
| DOI: | 10.1080/19932820.2020.1857525 |
| Popis: | Factor V Leiden G1691A (FVL) and Factor II prothrombin G20210A (PGM) mutations are the leading causes of thrombophilia. In this study, we have investigated the prevalence of the FVL G1691A and PGM G20210A single nucleotide polymorphisms (SNPs) among Libyan deep vein thrombosis (DVT) and myocardial infarction (MI) patients. SNP genotyping was performed using high-resolution melt analysis (HRM) and DNA sequencing. Biochemical parameters conducted on 112 males and 93 females showed no significant difference in means between the control group and the deep vein thrombosis and myocardial infarction groups. For Factor V Leiden, 40 samples were genotyped. Of the 40 samples, 6 (15.0%) of them were heterozygous and no one was homozygous. As for Factor II SNP, 59 samples were genotyped and only 2 (3.3%) were heterozygous. All the heterozygous samples showed 100% concordance between the HRM-PCR and DNA sequence analysis. Our study showed, for the first time, that both the FVL and PGM mutations are present among Libyan DVT and MI patients and that the FVL mutation is significantly associated with DVT but not with MI. However, our results do not support the association of PGM G20210A mutation with DVT or MI. |
| Databáze: | OpenAIRE |
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