Determination of immunoreactive antiarrhythmic peptide (AAP) in rats
| Autor: | Yusuke Kawahara, Shigeru Aonuma, Masaru Okabe, Yasuhiro Kohama, Tsutomu Mimura |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Male
medicine.medical_specialty Guinea Pigs Radioimmunoassay Peptide Polyethylene glycol Guinea pig chemistry.chemical_compound Fibrinolytic Agents Internal medicine medicine Animals Pharmacology chemistry.chemical_classification Kidney Molecular mass Myocardium Rats Inbred Strains Amino acid Rats medicine.anatomical_structure Endocrinology chemistry Sephadex Chromatography Gel Oligopeptides |
| Zdroj: | Journal of pharmacobio-dynamics. 8(12) |
| ISSN: | 0386-846X |
| Popis: | A sensitive and specific radioimmunoassay for antiarrhythmic peptide (AAP) has been developed, utilizing 3-(4-hydroxy, 5-[125I]iodophenyl)propionyl AAP and guinea pig anti-AAP serum. Synthetic AAP was used as a standard and the polyethylene glycol method was employed to separate free AAP from antibody-bound AAP. The minimum detectable dose of AAP was 0.2 pmol. In the assay system, immunoreactivity was shown not to be attributable to fragments derived from AAP, gelatin or collagen peptides, which sequences differ only by one amino acid from those of AAP. Immunoreactive (IR) AAP extracted from rat tissues and serum gave parallel dose-response curves to those of AAP. Thus, the AAP equivalents per g or ml of tissues measured in adult male rats were 203 pmol in heart, 166 pmol in kidney, 4 pmol in serum, and 2 to 6 pmol in the other tissues. IR-AAP was separated into two fractions by Sephadex G-25 chromatography. Fr. I was considered to have a larger molecular mass than AAP, while Fr. II appeared to have a molecular mass equal to AAP. Ratios of Fr. II in total IR-AAP were 50% in heart and 10% in kidney, while serum IR-AAP gave only Fr. II. The IR-AAP level in heart showed a positive correlation increase with age in rats. |
| Databáze: | OpenAIRE |
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