Matrigel induces thymosin beta 4 gene in differentiating endothelial cells
| Autor: | Derrick S. Grant, Maura C. Kibbey, A.L. Goldstein, Peter D. Burbelo, S. LaFlamme, Hynda K. Kleinman, James L. Kinsella |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
DNA
Complementary Endothelium Cellular differentiation Molecular Sequence Data Biology Transfection medicine Humans Cloning Molecular Basement membrane Tube formation Matrigel Base Sequence Cell Cycle Microfilament Proteins Thymosin Cell Differentiation Cell Biology Molecular biology Capillaries Extracellular Matrix Thymosin beta-4 Drug Combinations medicine.anatomical_structure Gene Expression Regulation Proteoglycans Collagen Endothelium Vascular Laminin hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Cell Science. 108:3685-3694 |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.108.12.3685 |
| Popis: | We performed differential cDNA hybridization using RNA from endothelial cells cultured for 4 hours on either plastic or basement membrane matrix (Matrigel), and identified early genes induced during the morphological differentiation into capillary-like tubes. The mRNA for one clone, thymosin beta 4, was increased 5-fold. Immunostaining localized thymosin beta 4 in vivo in both growing and mature vessels as well as in other tissues. Endothelial cells transfected with thymosin beta 4 showed an increased rate of attachment and spreading on matrix components, and an accelerated rate of tube formation on Matrigel. An antisense oligo to thymosin beta 4 inhibited tube formation on Matrigel. The results suggest that thymosin beta 4 is induced and likely involved in differentiating endothelial cells. Thymosin beta 4 may play a role in vessel formation in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|