Amycolatopsis suaedae sp. nov., an endophytic actinomycete isolated from Suaeda maritima roots
| Autor: | Kannika Duangmal, Atsuko Matsumoto, Paweena Suksaard, Tanatorn Chantavorakit |
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| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial 0106 biological sciences 0301 basic medicine Arabinose Amycolatopsis Chenopodiaceae Diaminopimelic Acid Plant Roots 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Suaeda maritima RNA Ribosomal 16S Botany Phylogeny Ecology Evolution Behavior and Systematics Mycelium Base Composition Strain (chemistry) biology Phylogenetic tree Fatty Acids Nucleic Acid Hybridization Vitamin K 2 Sequence Analysis DNA General Medicine Thailand 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques Actinobacteria genomic DNA 030104 developmental biology chemistry |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 69:2591-2596 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.003546 |
| Popis: | An actinomycete strain, designated 8-3EHSuT, was isolated from surface-sterilised roots of Suaeda maritima, collected from Petchburi province, Thailand. Taxonomic position of strain 8-3EHSuT was studied using a polyphasic approach. Phylogenetic determination based on the 16S rRNA gene sequence similarity showed that strain 8-3EHSuT belongs to the genus Amycolatopsis , with the highest sequence similarity to Amycolatopsis jiangsuensis KLBMP 1262T (96.9 %). The colony of strain 8-3EHSuT was yellowish white. Long straight mycelium breaking down into fragments were observed. Growth occurred at temperatures 15–45 °C and at pH 6.0–10.0. The strain contained meso-diaminopimelic acid, arabinose, galactose and mannose in whole-organism hydrolysates. The predominant menaquinone was MK-9(H4). The major fatty acids were C16 : 0, iso-C16 : 0 and iso-C15 : 0. Polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, two unknown amino lipids and an unknown lipid. The G+C content of genomic DNA was 71.8 mol%. A phylogenetic tree based on 16S rRNA sequences showed that the strain 8-3EHSuT formed a distinct evolutionary linage within the genus Amycolatopsis . Based on analysis results of physiological, biochemical and chemotaxonomic data, strain 8-3EHSuT represents a novel species of the genus Amycolatopsis , for which the name Amycolatopsis suaedae sp. nov. is proposed. The type strain is 8-3EHSuT (=TBRC 8488T=NBRC 113449T). |
| Databáze: | OpenAIRE |
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