Persistent Changes in Gene Expression Induced by Estrogen and Progesterone in the Rat Mammary Gland
| Autor: | Melanie R. Ginger, Maria F. Gonzalez-Rimbau, Jason P. Gay, Jeffrey M. Rosen |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
RNA Untranslated medicine.drug_class medicine.medical_treatment Molecular Sequence Data Mammary gland Biology Chromatin remodeling Mammary Glands Animal Endocrinology Pregnancy Internal medicine Gene expression medicine Animals Epigenetics Cloning Molecular Rats Wistar Molecular Biology In Situ Hybridization Progesterone Regulation of gene expression Estradiol Nuclear Proteins Reproducibility of Results General Medicine Blotting Northern Prolactin Rats Up-Regulation Steroid hormone medicine.anatomical_structure Gene Expression Regulation Estrogen Perphenazine Female Carrier Proteins |
| Zdroj: | Molecular Endocrinology. 15:1993-2009 |
| ISSN: | 1944-9917 0888-8809 |
| DOI: | 10.1210/mend.15.11.0724 |
| Popis: | Epidemiological studies have consistently shown that an early full-term pregnancy is protective against breast cancer. We hypothesize that the hormonal milieu that is present during pregnancy results in persistent changes in the pattern of gene expression in the mammary gland, leading to permanent changes in cell fate that determine the subsequent proliferative response of the gland. To investigate this hypothesis, we have used suppression subtractive hybridization to identify genes that are persistently up-regulated in the glands of E- and progesterone (P)-treated Wistar-Furth rats 28 d after steroid hormone treatment compared with age-matched virgins. Using this approach, a number of genes displaying persistent altered expression in response to previous treatment with E and P were identified. Two markers have been characterized in greater detail: RbAp46 and a novel gene that specifies a noncoding RNA (designated G.B7). Both were persistently up-regulated in the lobules of the regressed gland and required previous treatment with both E and P for maximal persistent expression. RbAp46 has been implicated in a number of complexes involving chromatin remodeling, suggesting a mechanism whereby epigenetic factors responsible for persistent changes in gene expression may be related to the determination of cell fate. These results provide the first support at the molecular level for the hypothesis that hormone-induced persistent changes in gene expression are present in the involuted mammary gland. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|