DNA microarray of global transcription factor mutant reveals membrane-related proteins involved in n-butanol tolerance in Escherichia coli
| Autor: | Ye Ni, Guo Chao Xu, Hai Ming Si, Rui Zhi Han, Fa Zhang, An Ning Wu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Butanol tolerance Mutant Management Monitoring Policy and Law Biology medicine.disease_cause Applied Microbiology and Biotechnology Transcriptome 03 medical and health sciences chemistry.chemical_compound Gene expression Membrane-related proteins medicine Escherichia coli Inner membrane Global transcription machinery engineering Gene Transcription factor Renewable Energy Sustainability and the Environment Butanol Research 030104 developmental biology General Energy Biochemistry chemistry lipids (amino acids peptides and proteins) DNA microarrays Biotechnology |
| Zdroj: | Biotechnology for Biofuels |
| ISSN: | 1754-6834 |
| Popis: | Background Escherichia coli has been explored as a platform host strain for biofuels production such as butanol. However, the severe toxicity of butanol is considered to be one major limitation for butanol production from E. coli. The goal of this study is therefore to construct butanol-tolerant E. coli strains and clarify the tolerance mechanisms. Results A recombinant E. coli strain harboring σ70 mutation capable of tolerating 2 % (v/v) butanol was isolated by the global transcription machinery engineering (gTME) approach. DNA microarrays were employed to assess the transcriptome profile of butanol-tolerant strain B8. Compared with the wild-type strain, 329 differentially expressed genes (197 up-regulated and 132 down-regulated) (p |
| Databáze: | OpenAIRE |
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