A simple liquid chromatography coupled to quadrupole time of flight mass spectrometry method for macrolide determination in tilapia fillets
| Autor: | Marcela Sismotto, Juliana A. Teles, Renata Antunes Estaiano De Rezende, Jonas Augusto Rizzato Paschoal, Felix Guillermo Reyes Reyes |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Analyte
Josamycin Analytical chemistry Liquid chromatography Aquaculture Tylosin Veterinary drug residues Mass spectrometry Food safety LC–QToF chemistry.chemical_compound medicine Sample preparation Tilmicosin Food contaminants Residue (complex analysis) Chromatography Food analysis ESPECTROMETRIA DE MASSAS Antimicrobial residues Hexane chemistry Macrolides Food Science medicine.drug Tilapia |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0889-1575 |
| DOI: | 10.1016/j.jfca.2014.02.006 |
| Popis: | A method for the identification and quantification of macrolides (erythromycin, josamycin, tilmicosin, tylosin, spiramycin and neospiramycin) in tilapia fillets by liquid chromatography coupled to quadrupole time of flight mass (LC–QToF) spectrometry is presented. Sample preparation was quite simple and low cost: proteins were precipitated and the analytes were extracted with ethanol, extract was defatted with hexane and concentrated by solvent evaporation. The matrix effect was statistically demonstrated during method validation, in which matrix-matched calibration was applied. Matrix effect mechanism was clarified thanks to the capability of QToF mass spectrometer of generating full scan spectra with accurate mass measurement. The limits of quantification were at least 45% lower than the maximum residue limits. The method was able to identify the studied macrolides with relative m/z errors lower than 2.5 ppm and to monitor two fragment ions per analyte, which is in accordance with the European Community recommendations for the analysis of contaminants in foods. Samples from the retail market of Sao Paulo State, Brazil, were analyzed by the developed method and none of them presented positive results for the macrolides studied. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|