Additional file 3 of Overexpression of a methyl-CpG-binding protein gene OsMBD707 leads to larger tiller angles and reduced photoperiod sensitivity in rice

Autor: Mengyu Qu, Zhujian Zhang, Tingmin Liang, Peipei Niu, Mingji Wu, Wenchao Chi, Chen, Zi-Qiang, Chen, Zai-Jie, Shubiao Zhang, Chen, Songbiao
Rok vydání: 2021
DOI: 10.6084/m9.figshare.14059510
Popis: Additional file 3: Figure S1. Detection of OsMBD707 transcript.(A) Schematic diagrams of predicted OsMBD707 splicing variants. (B) Sequence alignment of predicted OsMBD707 splicing variants. Sequences for primers designed to distinguish the two predicted alternative transcripts (predicted amplification sizes of 106 bp and 94 bp for XM_015764399.1/LOC_Os12g42550.1 and XM_015764400.2/LOC_Os12g42550.2, respectively.) are indicated by arrow. (C) RT-PCR analysis for detecting predicted OsMBD707 splicing variants. Only an amplification size of 106 bp for XM_015764399.1/LOC_Os12g42550.1 was detected in the roots, stems, leaves, spikelets, seeds, and panicle axes. (D) Sequencing confirmation of the 106 bp-amplified product of XM_015764399.1/LOC_Os12g42550.1. (PPT 2129 kb)
Databáze: OpenAIRE