Activity-wheel running attenuates suppression of natural killer cell activity after footshock
| Autor: | Shawn D. Youngstedt, H. S. Yoo, J. M. Warren, J. L. Meyerhoff, Rod K. Dishman, B. N. Bunnell, Liliana Jaso-Friedmann, E. H. Mougey, Donald L. Evans |
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| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty Physiology Lymphocyte Physical Exertion Radioimmunoassay Citrate (si)-Synthase Adrenocorticotropic hormone Biology Natural killer cell Random Allocation chemistry.chemical_compound Adrenocorticotropic Hormone Corticosterone Physiology (medical) Internal medicine medicine Animals Cytotoxic T cell Lymphocyte Count Muscle Skeletal Shock Flow Cytometry Rats Inbred F344 Prolactin Rats Killer Cells Natural Endocrinology medicine.anatomical_structure chemistry Spleen psychological phenomena and processes Glucocorticoid Follow-Up Studies medicine.drug |
| Zdroj: | Journal of Applied Physiology. 78:1547-1554 |
| ISSN: | 1522-1601 8750-7587 |
| DOI: | 10.1152/jappl.1995.78.4.1547 |
| Popis: | We studied whether voluntary running in an activity wheel moderates splenic natural killer (NK) cell cytotoxicity after footshock. Young (50-day) male Fischer 344 rats were randomly assigned to 1) sedentary (n = 16) or 2) activity-wheel (n = 16) groups that each received controllable or uncontrollable footshock on 2 consecutive days or 3) a sedentary home-cage control group (n = 8). Spleens and trunk blood were collected 30 min after the second footshock session. Cytotoxicity was determined by a standard 4-h 51Cr release assay. Percentages of OX6+ (B), OX8+ [T suppressor/cytotoxic (Ts/c)], W3/25+ (T helper), Thy-1.1 (Pan T cell marker), and 5C6+ (NK) cells were determined by flow cytometry. Plasma adrenocorticotropic hormone, corticosterone, and prolactin concentrations were measured by radioimmunoassay as modulators of NK activity. Percentage of specific lysis after footshock was approximately 52% of control values for sedentary animals compared with approximately 96% of control values for activity-wheel animals. The groups did not differ in percentages of NK or Ts/c cells. We conclude that voluntary activity-wheel running protects against the suppression of splenic NK activity induced by footshock. This protective effect of wheel running is not explained by an elevation in baseline NK activity; increased percentages of splenic NK or Ts/c cells; or plasma levels of adrenocorticotropic hormone, corticosterone, and prolactin. |
| Databáze: | OpenAIRE |
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