Helicobacter pylori upregulates matrilysin (MMP-7) in epithelial cells in vivo and in vitro in a Cag dependent manner
Autor: | Abed M. Zaitoun, John Atherton, Susan A. Watson, Neil Hand, Rachael J. Thomas, Hilary M. Collins, James R. Bebb, F Aviles, Darren P. Letley |
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Rok vydání: | 2003 |
Předmět: |
Male
Pathology medicine.medical_specialty Enzyme-Linked Immunosorbent Assay medicine.disease_cause Helicobacter Infections Bacterial Proteins medicine Gastric mucosa Humans Matrilysin Antrum Cells Cultured Aged biology Helicobacter pylori Reverse Transcriptase Polymerase Chain Reaction Stomach digestive oral and skin physiology Gastroenterology Nuclear Proteins Epithelial Cells Middle Aged bacterial infections and mycoses biology.organism_classification Molecular biology Immunohistochemistry digestive system diseases Up-Regulation Enzyme Activation medicine.anatomical_structure Real-time polymerase chain reaction Cell culture Gastric Mucosa Matrix Metalloproteinase 7 Female Carcinogenesis |
Zdroj: | Gut. 52(10) |
ISSN: | 0017-5749 |
Popis: | Background and aims: Matrix metalloproteinase-7 (MMP-7) is important in normal and pathological remodelling of epithelial-matrix interactions, and is upregulated in gastric cancer. Helicobacter pylori infection is the first stage in gastric carcinogenesis, and therefore our aim was to determine if H pylori upregulated gastric MMP-7 expression and if this was affected by strain virulence. Methods: We took gastric biopsy specimens at endoscopy from H pylori infected (n = 17) and uninfected (n = 18) patients and assessed MMP-7 expression by ELISA, real time polymerase chain reaction (PCR), and immunohistochemistry (concentrating on epithelial cells in the proliferative zone). We PCR typed H pylori for cagE and vacA. We performed H pylori/cell line coculture studies with wild-type pathogenic and non-pathogenic H pylori strains and with CagE− and VacA− isogenic mutants. Results: Gastric biopsy specimens from H pylori+ patients expressed higher levels of MMP-7 at the protein and mRNA levels in the antrum and corpus (for example, by ELISA: H pylori+ 0.182 OD units vH pylori− 0.059; p = 0.009 antrum). Epithelial cells from H pylori+ patients stained more intensely for MMP-7 than those from uninfected patients, including in the proliferative zone containing pluripotent cells (p |
Databáze: | OpenAIRE |
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