Both CD62 and CD162 antibodies prevent formation of CD36-dependent platelets, rosettes, and artefactual pseudoexpression of platelet markers on white blood cells: a study with ImageStream®

Autor: Chantal Jayat-Vignoles, Jean Feuillard, Magali Donnard, Catherine Ouk
Přispěvatelé: Physiologie Moléculaire de la Réponse Immune et des Lymphoproliférations (PMRIL), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Centre National de la Recherche Scientifique (CNRS), Service d'Hématologie biologique [CHU Limoges], CHU Limoges
Jazyk: angličtina
Rok vydání: 2011
Předmět:
CD36 Antigens
Male
P-selectin
Platelet Aggregation
MESH: Membrane Glycoproteins
MESH: Flow Cytometry
MESH: Antibodies
Monoclonal
0302 clinical medicine
Leukocytes
MESH: Microscopy
Confocal
Platelet
MESH: Blood Platelets
Whole blood
MESH: Platelet Aggregation
0303 health sciences
Membrane Glycoproteins
Microscopy
Confocal
MESH: Middle Aged
medicine.diagnostic_test
Immunochemistry
Antibodies
Monoclonal
Middle Aged
Flow Cytometry
3. Good health
P-Selectin
medicine.anatomical_structure
MESH: Artifacts
MESH: P-Selectin
[SDV.IMM]Life Sciences [q-bio]/Immunology
Female
Artifacts
Adult
Blood Platelets
Histology
medicine.drug_class
Biology
Monoclonal antibody
Pathology and Forensic Medicine
Flow cytometry
MESH: Leukocytes
03 medical and health sciences
medicine
Humans
MESH: Platelet Activation
Platelet activation
030304 developmental biology
MESH: Humans
MESH: Antigens
CD36
MESH: Immunochemistry
Monocyte
MESH: Biological Markers
MESH: Adult
Cell Biology
Platelet Activation
Molecular biology
MESH: Male
Cytometry
MESH: Female
Biomarkers
030215 immunology
Zdroj: Cytometry Part A
Cytometry Part A, Wiley, 2011, 79 (6), pp.477-84. ⟨10.1002/cyto.a.21050⟩
ISSN: 1552-4922
1552-4930
DOI: 10.1002/cyto.a.21050⟩
Popis: International audience; Fluorescent labeled monoclonal antibodies (mAbs) against CD36 are routinely used as monocyte, erythroid, or platelet markers in clinical cytometry. CD36 has recently been proposed by various authors as a valuable marker helping to enumerate leukocyte's subpopulations by flow cytometry. However, it is known that binding of CD36 may induce platelet activation and formation of platelet's rosettes on leukocytes, resulting in false expression of platelet markers on white blood cells. To study this phenomenon, we have combined classical flow cytometry and a new quantitative flow imaging technique with the ImageStream(®) analyzer. We show that CD36 ligation induces activation of platelets with CD62 expression and their adhesion on leukocytes due to CD62 and CD162 interactions. Preincubation of whole blood samples with either anti-CD62 or anti-CD162 antibodies could prevent formation of these rosettes. Our approach also emphasizes the fact that immunomorphological analysis of cell events with ImageStream technology is a useful tool to validate the specificity of marker's labeling or to elucidate incoherent results obtained with classical flow cytometry. We thus propose to prevent false platelet labeling on leukocytes by preincubation with either anti-CD62 or anti-CD162 antibodies when using CD36 mAbs.
Databáze: OpenAIRE
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