Idebenone improves quality of ram sperm by mitigating oxidative stress during cryopreservation
| Autor: | Hilal Musadiq Khan, Mehrajuddin Naikoo, Riaz Ahmad Shah, Arjuma Khatun, Azad Ahmad Ahanger, F. A. Lone, Zahoor Ahmad Pampori |
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| Rok vydání: | 2019 |
| Předmět: |
Male
endocrine system Ubiquinone Semen General Biochemistry Genetics and Molecular Biology Cryopreservation Antioxidants law.invention Lipid peroxidation Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cryoprotective Agents law Malondialdehyde Freezing medicine Idebenone Animals Humans Acrosome 030219 obstetrics & reproductive medicine Sheep urogenital system Extender 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Sperm Spermatozoa Semen Analysis Oxidative Stress chemistry Sperm Motility Lipid Peroxidation General Agricultural and Biological Sciences medicine.drug Semen Preservation |
| Zdroj: | Cryobiology. 90 |
| ISSN: | 1090-2392 |
| Popis: | The present study was designed to test the effect of different levels of idebenone, a potent antioxidant on the quality of ram semen at post thaw. Eighteen (18) ejaculates were collected and extended with tris extender supplemented with no antioxidant (CON), with 2 μM idebenone (Id2), 5 μM idebenone (Id5), 7.5 μM idebenone (Id7.5) and 10 μM idebenone (Id10). The sperm quality was determined in terms of percent sperm motility, live sperm percentage, percent hypoosmotic swelling test (HOST) positive spermatozoa and percent intact acrosome (PIA). Moreover, malondialdehyde (MDA) level, an end product of lipid peroxidation (LPO) was also measured at post thaw both in seminal plasma and sperm cell. At post thaw, the percent sperm motility was significantly higher (p 0.05) for Id10 as compared to Id2, Id5, Id7.5 and control. The live sperm percentage was non-significantly (p 0.05) higher for Id10 as compared to control, Id5 and Id7.5 but significantly higher than Id2. The percent HOST positive spermatozoa was significantly higher (p 0.05) for Id10 than control, Id2 and Id5. The MDA level in seminal plasma was significantly lower (p 0.05) for Id10 than control and Id2. The MDA level in spermatozoa did show similar trend as in seminal plasma. Further, all the sperm parameters at all idebenone levels declined significantly from pre freeze to post thaw. In conclusion, idebenone at 10 μM level improved post thaw sperm quality by mitigating peroxidative stress, hence could be considered as a promising antioxidant additive for cryopreservation of ram semen. |
| Databáze: | OpenAIRE |
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