Identification of FtsW and characterization of a new ftsW division mutant of Escherichia coli
| Autor: | William D. Donachie, K J Begg, M M Khattar |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cell division
Mutant Molecular Sequence Data Biology Microbiology Eukaryotic translation Suppression Genetic Bacterial Proteins Protein biosynthesis Escherichia coli FtsZ Cytoskeleton Overproduction Molecular Biology Alleles Microscopy Base Sequence Cell growth Escherichia coli Proteins Membrane Proteins Sequence Analysis DNA Molecular biology Cytoskeletal Proteins Phenotype Protein Biosynthesis Mutation biology.protein bacteria biological phenomena cell phenomena and immunity Cell Division Research Article |
| Zdroj: | Journal of bacteriology. 176(23) |
| ISSN: | 0021-9193 |
| Popis: | The product of the ftsW gene has been identified as a polypeptide that, like the related RodA protein, shows anomalous mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. FtsW is produced at low levels that can be increased by altering the translation initiation region of the mRNA. Overproduction of FtsW strongly inhibits cell growth. A new mutant allele, ftsW201, causes a temperature-dependent block in the initiation stage of cell division which is similar to the division block in ftsZ mutants. The block in initiation of division in the ftsW201 allele is shown to be independent of FtsZ or the FtsZ inhibitor, SulA. In addition, the ftsW201 mutant is hypersensitive to overproduction of the division initiation protein FtsZ at the permissive temperature. Our results suggest a role for FtsW in an early stage of division which may involve an interaction with FtsZ. |
| Databáze: | OpenAIRE |
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