Endothelial damage and vascular calcification in patients with chronic kidney disease
| Autor: | Pedro Aljama, Francisco Triviño, Marina Álvarez-Benito, Julia Carracedo, Alejandro Martin-Malo, Mariano Rodriguez, Andrés Carmona, Rafael Ramírez, Maria-Antonia Alvarez-Lara, Sagrario Soriano |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Physiology Primary Cell Culture Monocytes Cell-Derived Microparticles Internal medicine medicine Human Umbilical Vein Endothelial Cells Humans In patient Annexin A5 Renal Insufficiency Chronic Vascular calcification Aged business.industry Stem Cells Calcinosis Fibroblasts Middle Aged medicine.disease Surgery Capillaries Platelet Endothelial Cell Adhesion Molecule-1 Cardiology Female Endothelium Vascular Complication business Kidney disease |
| Zdroj: | American journal of physiology. Renal physiology. 307(11) |
| ISSN: | 1522-1466 |
| Popis: | Vascular calcification (VC) is a frequent complication of chronic kidney disease (CKD) and is a predictor of cardiovascular morbidity and mortality. In the present study, we investigated the potential involvement of endothelial microparticles (MPs) and endothelial progenitor cells (EPCs) in the generation of VC in CKD patients. The number of circulating EMPs is greater in patients with VC than without VC (307 ± 167 vs. 99 ± 75 EMPs/μl, P < 0.001). The percentage of EPCs is significantly lower in patient with VC than in patients without VC (0.14 ± 0.11% vs. 0.25 ± 0.18%, P = 0.002). The number of EPCs expressing osteocalcin (OCN) was higher in VC patients (349 ± 63 cells/100,000) than in non-VC patients (139 ± 75 cells/100,000, P < 0.01). In vitro, MPs obtained from CKD patients were able to induce OCN expression in EPCs from healthy donors; the increase in OCN expression was more accentuated if MPs were obtained from CKD patients with VC. MPs from CKD patients also induced OCN expression in vascular smooth muscle cells and fibroblasts. In CKD patients, the rise in endothelial MPs associated with a decrease in the number of EPCs, suggesting an imbalance in the processes of endothelial damage and repair in CKD patients, mainly those with VC. Our results suggest that EPCs, through OCN expression, may directly participate in the process of VC. |
| Databáze: | OpenAIRE |
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