Properties of intact and univalent (Fab) antibodies raised against isolated, solubilized, mouse zonae pellucidae
| Autor: | D. W. Richardson, E. Holme, R. J. Aitken, M. Hulme |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
endocrine system Embryology Antibody Affinity Immunoglobulin Fab Fragments Mice chemistry.chemical_compound Endocrinology Human fertilization Antigen Antibody Specificity medicine Animals education Zona Pellucida reproductive and urinary physiology Ovum Sperm-Ovum Interactions Antiserum education.field_of_study biology urogenital system Immunogenicity Obstetrics and Gynecology Cell Biology Sperm receptor Trypsin Solubility Reproductive Medicine chemistry Biochemistry Antibody Formation embryonic structures Urea biology.protein Female gamma-Globulins Antibody medicine.drug |
| Zdroj: | Reproduction. 66:327-334 |
| ISSN: | 1741-7899 1470-1626 |
| Popis: | Summary. Intact and univalent antibodies were prepared against mechanically isolated mouse zonae pellucidae solubilized in a variety of ways (heat, low pH, SDS, urea and trypsin). The antisera bound avidly and specifically to solubilized iodinated zona antigens and the intact zona structure. When the concentrations of immunoreactive Fab material in the intact and univalent antibody preparations were equalized and compared for their ability to block the sperm-binding stage of fertilization, only the intact \g=g\-globulinpreparations possessed antifertility activity. These results indicate that antibodies raised against intact solubilized zonae pellucidae block fertilization by cross-linking antigens on the outer zona surface, thereby indirectly masking the sperm receptor sites. The integrity of these surface components did not appear to be affected by solubilization procedures that disrupt non-covalent bonds (heating, low pH, SDS and urea) although they did appear to be adversely affected by trypsin treatment. None of the antisera tested contained antibodies directed against the sperm receptor site indicating that these critical components lack immunogenicity. |
| Databáze: | OpenAIRE |
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