Generation and fluorescent in situ hybridization mapping of yeast artificial chromosomes of 1p, 17p, 17q, and 19q from a hybrid cell line by high-density screening of an amplified library
| Autor: | G.J.B. van Ommen, Kenneth H. Fischbeck, E. J. Meershoek, K.L. Chen, M.S. Driesen, P. Mollevanger, Martin C. Wapenaar, J. G. Dauwerse |
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| Rok vydání: | 1991 |
| Předmět: |
Yeast artificial chromosome
congenital hereditary and neonatal diseases and abnormalities Alu element chemical and pharmacologic phenomena Molecular cloning Biology Hybrid Cells Polymerase Chain Reaction Nucleic acid thermodynamics Gene mapping hemic and lymphatic diseases Genetics Humans Genomic library Cloning Molecular Gene Library Repetitive Sequences Nucleic Acid Genome Human Gene Amplification Chromosome Chromosome Mapping Nucleic Acid Hybridization Molecular biology DNA Fingerprinting Chromosome 17 (human) Microscopy Fluorescence Chromosomes Human Pair 1 Chromosomes Fungal Chromosomes Human Pair 19 Chromosomes Human Pair 17 |
| Zdroj: | Genomics. 11(4) |
| ISSN: | 0888-7543 |
| Popis: | A yeast artificial chromosome (YAC) library has been constructed from a somatic cell hybrid containing a t(1p;19q) chromosome and chromosome 17. After amplification, part of this library was analyzed by high-density colony filter screening with a repetitive human DNA probe (Alu). The human YACs distinguished by the screening were further analyzed by Alu fingerprinting and Alu PCR. Fluorescent in situ hybridization (FISH) was performed to localize the YACs to subchromosomal regions of chromosome 1p, 17, or 19q. We have obtained a panel of 123 individual YACs with a mean size of 160 kb, and 77 of these were regionally localized by FISH: 33 to 1p, 10 to 17p, 25 to 17q, and 9 to 19q. The YACs cover a total of 19.7 Mb or 9% of the 220 Mb of human DNA contained in the hybrid. No overlapping YACs have yet been detected. These YACs are available upon request and should be helpful in mapping studies of disease loci, e.g., Charcot-Marie-Tooth disease, Miller-Dieker syndrome, hereditary breast tumor, myotonic dystrophy, and malignant hyperthermia. |
| Databáze: | OpenAIRE |
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