Mass spectrometry imaging with laser-induced postionization
| Autor: | Klaus Dreisewerd, Johannes Müthing, Marcel Wiegelmann, Jens Soltwisch, Simeon Vens-Cappell, Hans Kettling |
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| Rok vydání: | 2015 |
| Předmět: |
Male
MALDI imaging Matrix-assisted laser desorption electrospray ionization Swine Carbohydrates Mass spectrometry Mass spectrometry imaging Atmospheric-pressure laser ionization law.invention Membrane Lipids Optics law Cerebellum Gangliosides Animals Ambient ionization Ions Multidisciplinary business.industry Chemistry Lasers Vitamins Seminiferous Tubules Laser Lipids Ion source Mice Inbred C57BL Solubility Rats Inbred Lew Malus Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Female Protons Atomic physics business |
| Zdroj: | Science. 348:211-215 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.aaa1051 |
| Popis: | Imaging lipid composition Chemical imaging of cell membranes can be performed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI), but low ionization efficiency often leads to a signal dominated by the main lipid components, such as abundant phosphatidylcholine species. Soltwisch et al. used a tunable laser for post-ionization of neutral species to boost the signal for other membrane components, such as cholesterol and phospho- and glycolipids. Imaging of cells and tissues with these methods allows differentiation based on a more extensive chemical signature. Science , this issue p. 211 |
| Databáze: | OpenAIRE |
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