Oxidation state specific analysis of arsenic species in tissues of wild-type and arsenic (+ 3 oxidation state) methyltransferase-knockout mice
Autor: | Miroslav Stýblo, Jenna M. Currier, Christelle Douillet, Zuzana Drobná |
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Rok vydání: | 2016 |
Předmět: |
Male
0301 basic medicine Environmental Engineering Methyltransferase Adipose tissue chemistry.chemical_element 010501 environmental sciences 01 natural sciences Article Arsenicals Arsenic Mice 03 medical and health sciences chemistry.chemical_compound medicine Animals Environmental Chemistry 0105 earth and related environmental sciences General Environmental Science Arsenite Kidney Chemistry business.industry Wild type Methyltransferases General Medicine Methylation Molecular biology Biotechnology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Knockout mouse business Oxidation-Reduction Water Pollutants Chemical |
Zdroj: | Journal of Environmental Sciences. 49:104-112 |
ISSN: | 1001-0742 |
DOI: | 10.1016/j.jes.2016.06.018 |
Popis: | Arsenic methyltransferase (As3mt) catalyzes the conversion of inorganic arsenic (iAs) to its methylated metabolites, including toxic methylarsonite (MAsIII) and dimethylarsinite (DMAsIII). Knockout (KO) of As3mt was shown to reduce the capacity to methylate iAs in mice. However, no data are available on the oxidation states of As species in tissues of these mice. Here, we compare the oxidation states of As species in tissues of male C57BL/6 As3mt-KO and wild-type (WT) mice exposed to arsenite (iAsIII) in drinking water. WT mice were exposed to 50 mg/L As and As3mt-KO mice that cannot tolerate 50 mg/L As were exposed to 0, 15, 20, 25 or 30 mg/L As. iAsIII accounted for 53% to 74% of total As in liver, pancreas, adipose, lung, heart, and kidney of As3mt-KO mice; tri- and pentavalent methylated arsenicals did not exceed 10% of total As. Tissues of WT mice retained iAs and methylated arsenicals: iAsIII, MAsIII and DMAsIII represented 55%–68% of the total As in the liver, pancreas, and brain. High levels of methylated species, particularly MAsIII, were found in the intestine of WT, but not As3mt-KO mice, suggesting that intestinal bacteria are not a major source of methylated As. Blood of WT mice contained significantly higher levels of As than blood of As3mt-KO mice. This study is the first to determine oxidation states of As species in tissues of As3mt-KO mice. Results will help to design studies using WT and As3mt-KO mice to examine the role of iAs methylation in adverse effects of iAs exposure. |
Databáze: | OpenAIRE |
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