Biomimetic synthesis of silver nanoparticles using Matricaria chamomilla extract and their potential anticancer activity against human lung cancer cells
| Autor: | Mehdi Dadashpour, Mohammad Pourhassan-Moghaddam, Mohammad Nouri, Nosratollah Zarghami, Narges Soozangar, Akram Firouzi-Amandi, Mohammad Jafar Maleki, Younes Pilehvar-Soltanahmadi, Farhad Jeddi |
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| Rok vydání: | 2018 |
| Předmět: |
Lung Neoplasms
Silver Materials science Matricaria Cell Survival Metal Nanoparticles Bioengineering 02 engineering and technology Absorption (skin) Real-Time Polymerase Chain Reaction Silver nanoparticle Flow cytometry Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Biomimetics Biomimetic synthesis medicine Zeta potential Humans DAPI Aqueous solution medicine.diagnostic_test biology Plant Extracts Cell Cycle 021001 nanoscience & nanotechnology biology.organism_classification Matricaria chamomilla chemistry A549 Cells Mechanics of Materials 030220 oncology & carcinogenesis 0210 nano-technology Nuclear chemistry |
| Zdroj: | Materials Science and Engineering: C. 92:902-912 |
| ISSN: | 0928-4931 |
| Popis: | Herbs having various natural substances can be utilized for the biosynthesis of Silver nanoparticles (AgNPs) and act as a stable, reliable and biocompatible alternative instead of the current physical and chemical approaches. It has been reported that Matricaria chamomilla possesses unique properties, especially anti-cancerous effects. The objective of the current work was to assess the chemical characteristics and anticancer effects of biosynthesized AgNPs applying aqueous extracts of M. chamomilla against A549 lung cancer cells. UV–visible spectrum showed the maximum absorption of the biosynthesized AgNPs at 430 nm. The crystalline structure of biosynthesized AgNPs in optimal conditions was confirmed by XRD. Moreover, the presence of Ag as the ingredient element was exhibited via EDX analysis. FT-IR results also verified the AgNPs synthesis using a plant extract. The spherical shapes of the AgNPs with an average diameter size around 45.12 nm and a zeta potential value of −34 mV were characterized using DLS, and confirmed through FE-SEM and TEM. In vitro cytotoxicity assay using MTT revealed that the biosynthesized AgNPs exhibited a dose- and time- dependent cytotoxic effect against A549 lung cancer cells. Moreover, the apoptotic effects of the AgNPs were demonstrated using DAPI staining, real-time PCR and flow cytometry. According to these findings, using M. chamomilla in combination with AgNPs via green-synthesis approach may be an efficient strategy for effective treatment of lung cancer. |
| Databáze: | OpenAIRE |
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