Molecular sequencing technologies in the diagnosis and management of prosthetic joint infections
| Autor: | Emane Gamie, Dileep Karthikappallil, Eleftherios Tsiridis, Zakareya Gamie, Eustathios Kenanidis, Stavros Stamiris |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Technology medicine.medical_specialty Prosthesis-Related Infections Prosthetic joint macromolecular substances Sensitivity and Specificity Pathology and Forensic Medicine 03 medical and health sciences High morbidity 0302 clinical medicine Genetics Humans Medicine Intensive care medicine Molecular Biology Arthritis Infectious business.industry fungi High-Throughput Nucleotide Sequencing nutritional and metabolic diseases food and beverages Prosthetic joint infection 030104 developmental biology Metagenomics 030220 oncology & carcinogenesis Molecular Medicine Culture negative business |
| Zdroj: | Expert Review of Molecular Diagnostics. 22:603-624 |
| ISSN: | 1744-8352 1473-7159 |
| DOI: | 10.1080/14737159.2021.1894929 |
| Popis: | Prosthetic joint infections (PJIs) can be challenging to eradicate and have high morbidity and mortality. Current microbiology culture methods can be associated with a high false-negative rate of up to 50%. Early and accurate diagnosis is crucial for effective treatment, and negative results have been linked to a greater rate of reoperation.There has been increasing investigation of the use of next-generation sequencing (NGS) technology such as metagenomic shotgun sequencing to help identify causative organisms and decrease the uncertainty around culture-negative infections. The clinical importance of the organisms detected and their management, however, requires further study. The polymerase chain reaction (PCR) has shown promise, but in recent years multiple studies have reported similar or lower sensitivity for bacteria detection in PJIs when compared to traditional culture. Furthermore, issues such as high cost and complexity of sample preparation and data analysis are to be addressed before it can move further toward routine clinical practice.Metagenomic NGS has shown results that inspire cautious optimism - both in culture-positive and culture-negative cases of joint infection. Refinement of technique could revolutionize the way PJIs are diagnosed, managed, and drastically improve outcomes from this currently devastating complication. |
| Databáze: | OpenAIRE |
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