Expresión de aif, parp y mirnas mir-145, mir-210 y mir-486 asociados con apoptosis en el cuerpo cavernoso de ratas sometidas a modelo de alcoholismo crónico
| Autor: | Valéria Paula Sassoli Fazan, Jairo Pinheiro da Silva, Bruno Cezar Schimming, Maria de Fátima Tazima Sorita Galli, Silvio Tucci Júnior, Paulo Novais, Mucio Luiz de Assis Cirino, José Antônio Thomazini, Maria Fernanda Barbirato da Mata Tiezzi, Vagner Schiavoni Sarraipo, Marcelo Martinez, Luis Fernando Tirapelli, Isabella Stracieri Gula, Carlos Augusto Fernandes Molina, Daniela Pretti da Cunha Tirapelli, Fabianna de Oliveira Buono, Andressa Romualdo Rodrigues, Fermino Sanches Lizarte Neto, Camila Albuquerque Mello de Carvalho, Marina T. Durand |
|---|---|
| Přispěvatelé: | Universidade de São Paulo (USP), University of Ribeirão Preto, Universidade Estadual Paulista (Unesp), Universidade Federal de São Carlos (UFSCar), UNIMAR, Univ Ribeirao Preto, Univ Marilia |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Web of Science International Journal of Morphology v.38 n.6 2020 SciELO Chile CONICYT Chile instacron:CONICYT |
| Popis: | Made available in DSpace on 2021-06-25T12:24:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-01 Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The objective of this analysis was to evaluate the mechanism of apoptosis by the expression of AIF and PARP, as well as their regulatory microRNAs: miR-145, miR-210 and miR-486, in the corpus cavemosum of rats submitted to a semivoltmtary alcoholism model. For this study 24 Wistar rats were divided into two groups: control (C) and treated with 20 % ethanol (A) for seven weeks. The corpus cavernosum samples were prepared for immunohistochemical analysis of AIF and PARP protein expression, and microRNAs miR-145, miR-210, miR-486 gene expression in cavernous tissue was performed by real time PCR. The immunohistochemical analysis showed little nuclear positive labeling for the protein PARP and AIF in the corpus cavernosum of control and ethanol treated animals. After analysis of miR-145, -210 and -486 microRNA expression in the 12 animals studied, no results were found with significant statistical difference between the control and alcoholized groups. The expression ofAIF and PARP and their regulatory inicroRNAs involved in apoptotic process (miR-145, miR-210 and miR-486) were not altered in the corpus cavernosum of rats submitted to semivoltmtary alcoholism. Sao Paulo Univ USP, Med Sch Ribeirao Preto, Dept Surg & Anat, Ribeirao Preto, SP, Brazil Univ Ribeirao Preto, Dept Med, Ribeirao Preto, Brazil UNESP, Biosci Inst Botucatu, Dept Anat, Botucatu, SP, Brazil Univ Fed Sao Carlos, Dept Morphol & Pathol, Sao Carlos, SP, Brazil Univ Marilia, Dept Odontol, Marilia, SP, Brazil Univ Marilia, Postgrad Program Struct & Funct Interact Rehabil, Marilia, SP, Brazil UNESP, Biosci Inst Botucatu, Dept Anat, Botucatu, SP, Brazil |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|