Protective effect of active perfusion in porcine models of acute myocardial ischemia
| Autor: | Zhifu Mao, Baohui Liu, Shengjun Dong, Zanxiang Feng |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Pathology Swine Ischemia Coronary Artery Bypass Off-Pump Myocardial Ischemia Apoptosis Constriction Pathologic 030204 cardiovascular system & hematology Creatine Biochemistry Constriction 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Troponin I Genetics medicine Animals Molecular Biology business.industry Caspase 3 Myocardium animal model Articles medicine.disease active perfusion Perfusion Stenosis Disease Models Animal medicine.anatomical_structure Oncology chemistry Proto-Oncogene Proteins c-bcl-2 030220 oncology & carcinogenesis Cardiology Aortic pressure Molecular Medicine business acute myocardial ischemia shunt perfusion Artery |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | Mortality rates associated with off‑pump coronary artery bypass (CAB) are relatively high, as the majority of patients requiring CAB are at a high risk for cardiac events. The present study aimed to establish porcine models of acute myocardial ischemia, and evaluate the protective role of shunt and active perfusion. A total of 30 pigs were randomly assigned to five groups, as follows: i) Sham (control); ii) A1 (shunt; stenosis rate, 55%); iii) A2 (shunt; stenosis rate, 75%); iv) B1 (active perfusion; stenosis rate, 55%); and v) B2 (active perfusion; stenosis rate, 75%) groups. Aortic pressure (P0), left anterior descending coronary pressure (P1), and coronary effective perfusion pressure (P1/P0) were measured. The expression levels of tumor necrosis factor‑α (TNF‑α), cardiac troponin (cTnI), creatine kinase‑myocardial band (CK‑MB), interleukin (IL)‑6, IL‑10, B‑cell lymphoma 2 (Bcl‑2), and caspase‑3 were detected using enzyme‑linked immunosorbent assay or western blotting. The myocardial apoptosis rate was determined using the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Ischemia models with stenosis rates of 55 and 75% were successfully constructed following suturing of the descending artery. Compared with the control, the 55 and 75% stenosis groups demonstrated significantly decreased P1/P0, increased expression levels of TNF‑α, cTnI, CK‑MB, IL‑6, IL‑10 and caspase‑3, an increased rate of myocardial apoptosis, and a decreased expression level of anti‑apoptotic protein, Bcl‑2. At 30 min following successful establishment of the model (ST segment elevation to 1 mm), group B demonstrated significantly increased P1/P0, decreased expression levels of TNF‑α, cTnI, CK‑MB, IL‑6, IL‑10 and caspase‑3, a decreased rate of myocardial apoptosis, and an increased expression level of anti-apoptotic protein, Bcl‑2. Furthermore, the current study indicated that active perfusion was more efficacious in maintaining myocardial perfusion and alleviating ischemic injury when compared with traditional shunt perfusion. |
| Databáze: | OpenAIRE |
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