LAL test and RPT for endotoxin detection of CPT-11/DSPE-mPEG2000 nanoformulation: What if traditional methods are not applicable?
| Autor: | Chan Li, Yaling Gan, Jianqi Xue, Jing Xu, Xingjie Liang, Yanan Jin, Jiabei Sun, Kaiyuan Wang, Juanjuan Jia, Yaqin Shi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Drug Pharmaceutical drug media_common.quotation_subject medicine.medical_treatment Pharmaceutical Science 02 engineering and technology Pharmacology 03 medical and health sciences medicine Irinotecan Hydrochloride Original Research Article media_common Amphiphilic molecule Rabbit pyrogen test DSPE-mPEG2000 Chemistry Endotoxin detection lcsh:RM1-950 food and beverages 021001 nanoscience & nanotechnology Limulus amebocyte lysate assay Irinotecan hydrochloride 030104 developmental biology lcsh:Therapeutics. Pharmacology High dosage Limulus amebocyte lysate Chemotherapeutic drugs 0210 nano-technology Micelle |
| Zdroj: | Asian Journal of Pharmaceutical Sciences Asian Journal of Pharmaceutical Sciences, Vol 13, Iss 3, Pp 289-296 (2018) |
| ISSN: | 2221-285X 1818-0876 |
| Popis: | Graphical Abstract Endotoxin can be measured mainly by two methods: the Rabbit Pyrogen Test (RPT) and Limulus Amebocyte Lysate (LAL) assay. We found that a chemotherapeutic drug nanoformulation composed of irinotecan hydrochloride and an amphiphilic molecule DSPE-mPEG2000 can interfere with the LAL. Furthermore, the RPT results indicated that at a relatively high dosage, the drug irinotecan hydrochloride can induce a hypothermia effect which forces us to reduce the dose. So it is necessary to develop new methods for the detection of endotoxin. Unlabelled image Endotoxin detection is an important step in drug characterization. Herein we found that a chemotherapeutic drug nanoformulation composed of irinotecan hydrochloride (CPT-11) and an amphiphilic molecule DSPE-mPEG2000 can interfere with the limulus amebocyte lysate assay (LAL). Furthermore, the rabbit pyrogen test (RPT) results indicated that at a relatively high dosage, the drug irinotecan hydrochloride can induce a hypothermia effect which may render the RPT results ambiguous in determination of the safety of the drug formulation.Our findings demonstrate limitations of endotoxin detection in micellar drugs, and call for the necessity of developing reliable endotoxin detection methods that can overcome the interference of nanomaterials in order to better ensure the drug safety of patients in future pharmaceutical drug development. |
| Databáze: | OpenAIRE |
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