Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST)
| Autor: | Edroaldo Lummertz da Rocha, Christos Boutopoulos, Sébastien Talbot, Marcelo Falchetti, Katiane Roversi, Hamid Ebrahimi Orimi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Neurite
TRPV1 Neuropeptide Sensory system 02 engineering and technology Calcitonin gene-related peptide Biology Somatosensory system calcium kinetics Article 03 medical and health sciences Dorsal root ganglion TJ1-1570 medicine Mechanical engineering and machinery Electrical and Electronic Engineering 030304 developmental biology 0303 health sciences Mechanical Engineering laser-induced side transfer viability 021001 nanoscience & nanotechnology adult DRG neurons Cell biology medicine.anatomical_structure sensory neurons nervous system Control and Systems Engineering Neuron 0210 nano-technology transcriptome laser-assisted bioprinting |
| Zdroj: | Micromachines Volume 12 Issue 8 Micromachines, Vol 12, Iss 865, p 865 (2021) |
| ISSN: | 2072-666X |
| Popis: | Cell bioprinting technologies aim to fabricate tissuelike constructs by delivering biomaterials layer-by-layer. Bioprinted constructs can reduce the use of animals in drug development and hold promise for addressing the shortage of organs for transplants. Here, we sought to validate the feasibility of bioprinting primary adult sensory neurons using a newly developed laser-assisted cell bioprinting technology, known as Laser-Induced Side Transfer (LIST). We used dorsal root ganglion neurons (DRG cell bodies of somatosensory neurons) to prepare our bioink. DRG-laden- droplets were printed on fibrin-coated coverslips and their viability, calcium kinetics, neuropeptides release, and neurite outgrowth were measured. The transcriptome of the neurons was sequenced. We found that LIST-printed neurons maintain high viability (Printed: 86%, Control: 87% on average) and their capacity to release neuropeptides (Printed CGRP: 130 pg/mL, Control CGRP: 146 pg/mL). In addition, LIST-printed neurons do not show differences in the expressed genes compared to control neurons. However, in printed neurons, we found compromised neurite outgrowth and lower sensitivity to the ligand of the TRPV1 channel, capsaicin. In conclusion, LIST-printed neurons maintain high viability and marginal functionality losses. Overall, this work paves the way for bioprinting functional 2D neuron assays. |
| Databáze: | OpenAIRE |
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