Recombinant human thyrotropin stimulates thyroid angiogenesis in vivo
| Autor: | Karine Angioi-Duprez, Adrien Duprez, Rémy Sapin, Georges Weryha, Laurent Brunaud, F Barbé, Béatrice Marie, Mihaela Muresan, Marc Klein, Jean-Michel Vignaud, Jérôme Chatelin, Rasa Zarnegar |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Angiogenesis Endocrinology Diabetes and Metabolism Transplantation Heterologous Thyroid Gland Mice Nude Thyrotropin Stimulation Models Biological Neovascularization Mice Endocrinology In vivo Internal medicine medicine Animals Humans Neovascularization Pathologic business.industry Microcirculation Thyroid Immunohistochemistry Recombinant Proteins Transplantation medicine.anatomical_structure medicine.symptom business Immunostaining |
| Zdroj: | Thyroid : official journal of the American Thyroid Association. 16(6) |
| ISSN: | 1050-7256 |
| Popis: | Endogenous TSH and rhTSH stimulate thyroid growth by a direct effect on thyrocytes. Our hypothesis was that rhTSH may also stimulate thyroid angiogenesis.A normal human thyroid tissue sample was grafted into the epigastric area of 14 nude mice. Mice were divided in two groups of 7. The first group (treated mice) received rhTSH stimulation (0.014 UI/mouse/day for 3 weeks), while the second group (control mice) had saline. Histological study with special focus on vascular characteristics was performed by image analysis at day 21 for each graft. VEGF immunostaining score, determined by immunohistochemistry, was defined as the percentage of labeled thyrocytes score, plus an intensity score.Thyroid follicles showed signs of increased colloid re-uptake activity in rhTSH group within a larger surface area than controls (p0.01). Thyrocytes were taller in the rhTSH group (p0.01). The diameter of capillary vessels was larger and the microvessels expansion more important in the rhTSH group (p0.02). Relative capillary area, defined as the ratio between capillary area and follicular area, was also higher in the rhTSH group (p0.02). VEGF immunostaining score was increased in the rhTSH group (p0.01).rhTSH stimulates angiogenesis and local VEGF expression in normal human thyroid. |
| Databáze: | OpenAIRE |
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