Synthetic Oligopeptides Mimicking γ-Core Regions of Cysteine-Rich Peptides of Solanum lycopersicum Possess Antimicrobial Activity against Human and Plant Pathogens
| Autor: | Tatyana I. Odintsova, E. V. Kulakovskaya, Marina P. Slezina, E. A. Istomina, Tatiana Abashina |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Cryptococcus neoformans chemistry.chemical_classification Oligopeptide biology QH301-705.5 Chemistry Antimicrobial peptides human pathogens Human pathogen Peptide General Medicine biology.organism_classification Antimicrobial plant pathogens Microbiology Plant disease antimicrobial peptides Biochemistry Biology (General) plant immunity Molecular Biology Clavibacter michiganensis cysteine-rich peptides γ-core |
| Zdroj: | Current Issues in Molecular Biology Volume 43 Issue 3 Pages 87-1242 Current Issues in Molecular Biology, Vol 43, Iss 87, Pp 1226-1242 (2021) |
| ISSN: | 1467-3045 |
| DOI: | 10.3390/cimb43030087 |
| Popis: | Plant cysteine-rich peptides (CRPs) represent a diverse group of molecules involved in different aspects of plant physiology. Antimicrobial peptides, which directly suppress the growth of pathogens, are regarded as promising templates for the development of next-generation pharmaceuticals and ecologically friendly plant disease control agents. Their oligopeptide fragments are even more promising because of their low production costs. The goal of this work was to explore the antimicrobial activity of nine short peptides derived from the γ-core-containing regions of tomato CRPs against important plant and human pathogens. We discovered antimicrobial activity in peptides derived from the defensin-like peptides, snakins, and MEG, which demonstrates the direct involvement of these CRPs in defense reactions in tomato. The CRP-derived short peptides appeared particularly active against the gram-positive bacterium Clavibacter michiganensis, which causes bacterial wilt—opening up new possibilities for their use in agriculture to control this dangerous disease. Furthermore, high inhibitory potency of short oligopeptides was demonstrated against the yeast Cryptococcus neoformans, which causes serious diseases in humans, making these peptide molecules promising candidates for the development of next-generation pharmaceuticals. Studies of the mode of action of the two most active peptides indicate fungal membrane permeabilization as a mechanism of antimicrobial action. |
| Databáze: | OpenAIRE |
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