Salmonella typhimurium as a basis for a live oral Echinococcus granulosus vaccine
| Autor: | Gordon Dougan, María Moreno, C E Hormaeche, Hernan Carol, José A. Chabalgoity |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Salmonella typhimurium
Salmonella Cellular immunity T-Lymphocytes Antibodies Helminth Administration Oral Helminth genetics In Vitro Techniques Biology Lymphocyte Activation medicine.disease_cause Microbiology Dogs Echinococcosis medicine Animals Dog Diseases Echinococcus granulosus DNA Primers Vaccines Synthetic Base Sequence General Veterinary General Immunology and Microbiology Immunogenicity Public Health Environmental and Occupational Health Toxoid biology.organism_classification Virology Echinococcus Infectious Diseases Genes Bacterial Antigens Helminth Immunoglobulin G Humoral immunity biology.protein Molecular Medicine Antibody |
| Zdroj: | Vaccine. 19:460-469 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(00)00197-3 |
| Popis: | A live attenuated Salmonella typhimurium vaccine candidate, LVR01, was constructed by introducing a null deletion into the aroC gene of the parental canine S. typhimurium isolate, P228067. LVR01 was used to orally deliver to the canine immune system a fatty acid binding protein (FABP) from Echinococcus granulosus (EgDf1), as a fusion protein with fragment C (TetC) of tetanus toxin. Immunization studies demonstrated that live LVR01 is well tolerated by orally vaccinated dogs. There was no detectable shedding of the vaccine strain in the faeces 2 days after immunization. Humoral antibody responses were observed against Salmonella, TetC and EgDf1. Cellular responses were consistently detected against Salmonella and TetC. A cellular response against EgDf1 was also seen in a proportion of the LVR01 vaccinated dogs. We propose S. typhimurium LVR01 as a carrier for recombinant antigens and a vector for the construction of multivalent oral vaccines for dogs. |
| Databáze: | OpenAIRE |
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