Pharmacological Studies on 3-Formylamino-7-methylsulfonylamino-6-phenoxy-4H-1-benzopyran-4-one (T-614), a Novel Antiinflammatory Agent. 4th Communication: Inhibitory Effect on the Production of Interleukin-1 and Interleukin-6
| Autor: | Takihiro Inaba, Yukihiko Aikawa, Keiichi Tanaka, Keiko Asaoka, Chosaku Yoshida, Hiroki Kawasaki |
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| Rok vydání: | 1992 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide medicine.medical_treatment Indomethacin Pharmacology Monocytes Cell Line chemistry.chemical_compound Humans Medicine Benzopyrans Drug Interactions THP1 cell line Interleukin 6 Sulfonamides Leukemia biology Interleukin-6 business.industry Monocyte Anti-Inflammatory Agents Non-Steroidal Interleukin In vitro medicine.anatomical_structure Cytokine chemistry Cell culture Immunology biology.protein business Interleukin-1 |
| Zdroj: | Journal of Pharmacobio-Dynamics. 15:649-655 |
| ISSN: | 1881-1353 0386-846X |
| DOI: | 10.1248/bpb1978.15.649 |
| Popis: | In vitro effects of 3-formylamino-7-methylsulfonylamino-6-phenoxy-4H-1-benzopyran-4-on e (T-614), a novel antiinflammatory compound, on the production of interleukin-1 (IL-1) and/or interleukin-6 (IL-6) by human monocytes and the THP-1 cells of a human monocytic cell line, were examined. T-614 inhibited the release of immunoreactive IL-1 beta from these cells stimulated with lipopolysaccharides (LPS) in a dose-dependent manner (0.3-30 micrograms/ml). The release of IL-6 from THP-1 cells, as determined by the assays for its hepatocyte-stimulating activities and immunoreactivities, was inhibited by T-614 with the IC50 values of 2.0 and 6.6 micrograms/ml, respectively. Northern blotting analysis using LPS-stimulated THP-1 cells indicated that the inhibitory effect of T-614 on IL-1 beta production is caused by the suppression of IL-1 beta mRNA expression. The inhibition of cytokine production by T-614 may provide an important insight into the additional mechanisms contributing to its antiinflammatory activities. |
| Databáze: | OpenAIRE |
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