White Blood Cell Acid Hydrolases in Leukaemias, Mucopolysaccharidoses and Mannosidosis
| Autor: | S. Autio, B. Hultberg, B. Berg, P. A. ÖCkerman |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Adolescent Glycoside Hydrolases Chronic myelocytic leukaemia Mannosidosis Myeloblast White blood cell Leukocytes Methods medicine Humans Child Aged Glucuronidase chemistry.chemical_classification Lymphocytic leukaemia Chemistry Isoelectric focusing Hematology Hydrogen-Ion Concentration Middle Aged Mucopolysaccharidoses Galactosidases Leukemia Lymphoid Leukemia Myeloid Acute Hexosaminidases Enzyme medicine.anatomical_structure Biochemistry Leukemia Myeloid Child Preschool Chromatography Gel Female Isoelectric Focusing Mannose Glucosidases Carbohydrate Metabolism Inborn Errors |
| Zdroj: | Scandinavian Journal of Haematology. 10:265-272 |
| ISSN: | 0036-553X |
| DOI: | 10.1111/j.1600-0609.1973.tb00071.x |
| Popis: | Simple, synthetic substrates, commercially available, were used for assay of five acid hydrolases (β-galactosidase, β-glucosidase, α-mannosidase, N-acetyl-β-glucosaminidase, and β-glucuronidase) in human white blood cells from 23 controls and 25 patients with inborn storage disorders or leukaemia. Conditions for release of the enzyme activities, pH-dependency, and properties on gel filtration and isoelectric focusing are described. In chronic myelocytic leukaemia high activities of α-mannosidase were found, whereas the activities of all enzymes were low in chronic lymphocytic leukaemia. In acute myeloblast leukaemia enzyme activities varied. In two cases of mannosidosis very low values of α-mannosidase were found. |
| Databáze: | OpenAIRE |
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