Tumor necrosis factor alpha promoter polymorphism −308 G/A in Brazilian patients with systemic lupus erythematosus
| Autor: | Maria Tereza Cartaxo Muniz, Nadja Maria Jorge Asano, Paulo Roberto Eleutério de Souza, Helker Albuquerque da Silva, Maria de Mascena Diniz Maia, Hildson Dornelas Angelo |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Adolescent Genotype Immunology Population Polymorphism Single Nucleotide law.invention Young Adult immune system diseases law medicine Humans Lupus Erythematosus Systemic Immunology and Allergy Genetic Predisposition to Disease Promoter Regions Genetic skin and connective tissue diseases education Genotyping Alleles Polymerase chain reaction Autoimmune disease education.field_of_study Systemic lupus erythematosus biology Tumor Necrosis Factor-alpha General Medicine Middle Aged medicine.disease Molecular biology Case-Control Studies biology.protein Female Gene polymorphism Antibody Serositis Brazil |
| Zdroj: | Human Immunology. 73:1166-1170 |
| ISSN: | 0198-8859 |
| Popis: | Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of antibodies to components of the cell nucleus in association with a diverse array of clinical manifestations. Polymorphisms in cytokines genes may play an important role in the development and clinical manifestation. Due to this, there is a great interest in the identification of biomarkers that which could quantify the susceptibility and disease activity. A case–control study of 98 lupus cases and 76 lupus-free adults controls, was performed to analyze whether or not the polymorphism of the TNF-α gene promoter at positions −308 G/A would alter the risk for SLE and clinical manifestations. Genotyping was carried out by polymerase chain reaction, PCR products were digested by NcoI restriction enzyme and fractionated after on 2% Agarose gel and visualized posteriorly staining by ethidium bromide. There were significant differences in the distribution of the TNF-α gene polymorphism between the SLE and control groups. Individual carriers of the variant allele A had a 3.29 (95% CI: 1.7738–6.1325)-fold increased risk for SLE. Moreover, association was observed between SLE patients and serositis ( P = 0.0228). This study presents a preliminary evidence of association between TNF-α polymorphism and SLE susceptibility in the Northeast population from Brazil. |
| Databáze: | OpenAIRE |
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