PME and Other ESBL-Positive Multiresistant Pseudomonas aeruginosa Isolated from Hospitalized Patients in the Region of Kurdistan, Iraq
| Autor: | Mohammed Hassan Fatah, Corné H. W. Klaassen, Dik J. Kok, Sebastian van Burgh, Aryann R. Ganjo, Abdulkadir A Alnakshabandi, Dlnya Asad, Kim Lee Ng, Anette M. Hammerum, Isam Y. Mansoor, Wil H. F. Goessens, Delshad M. Maghdid |
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| Přispěvatelé: | Medical Microbiology & Infectious Diseases, Urology |
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Hospitalized patients Immunology Microbial Sensitivity Tests medicine.disease_cause Polymerase Chain Reaction Microbiology beta-Lactamases 03 medical and health sciences Bacterial Proteins Drug Resistance Multiple Bacterial Humans Medicine Pseudomonas Infections Amplified Fragment Length Polymorphism Analysis 030304 developmental biology Pharmacology Cross Infection 0303 health sciences 030306 microbiology Transmission (medicine) business.industry Pseudomonas aeruginosa Anti-Bacterial Agents Genes Bacterial Iraq Iraqi kurdistan business Polymorphism Restriction Fragment Length |
| Zdroj: | Microbial Drug Resistance. Mechanisms Epidemiology and Disease, 25(1), 32-38. Mary Ann Liebert Inc. |
| ISSN: | 1931-8448 1076-6294 |
| DOI: | 10.1089/mdr.2018.0036 |
| Popis: | Nosocomial infections occur worldwide and also in the Kurdistan region. Frequently patients colonized with multiresistant Pseudomonas aeruginosa isolates are encountered in many hospitals. As information is lacking with respect to the mechanisms of resistance responsible for the multiresistant character of the P. aeruginosa isolates and their genetic relationship, isolates were prospectively collected and characterized with respect to their mechanism of resistance. During 2012 and 2013, 81 P. aeruginosa isolates were collected from three teaching hospitals in the city of Erbil, Iraq. Susceptibility testing was performed using the VITEK-2 system. Isolates were screened for the presence of extended-spectrum β-lactamases (ESBLs) and for the presence of metallo β-lactamases (MBLs). The presence of serine carbapenemases was detected by PCR. The genetic relationship of the isolates was demonstrated by amplified fragment length polymorphism (AFLP). Susceptibility results revealed high rates of resistance against all classes of antibiotics except polymyxins. Genetic characterization demonstrated the presence of ESBL-genes, that is, bla |
| Databáze: | OpenAIRE |
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