Molecular cloning and characterization of an SRCAP chromatin remodeling homologue in Toxoplasma gondii
| Autor: | Peter Yaciuk, John C. Chrivia, Karuna Nallani, William J. Sullivan, M. Alexandra Monroy, Wolfgang Bohne, Sherry F. Queener, Charles K. Smith |
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| Rok vydání: | 2003 |
| Předmět: |
Transcription
Genetic Genes Protozoan Molecular Sequence Data Molecular cloning Biology Chromatin remodeling Homology (biology) Complementary DNA Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Gene Adenosine Triphosphatases Genetics Sequence Homology Amino Acid General Veterinary Intron Nuclear Proteins General Medicine CREB-Binding Protein Chromatin Open reading frame Infectious Diseases Gene Expression Regulation Insect Science Trans-Activators Parasitology Toxoplasma HeLa Cells |
| Zdroj: | Parasitology Research. 90:1-8 |
| ISSN: | 1432-1955 0932-0113 |
| DOI: | 10.1007/s00436-002-0814-1 |
| Popis: | We have identified and mapped a gene in Toxoplasma gondii that encodes a homologue of SRCAP (Snf2-related CBP activator protein), a member of the SNF/SWI family of chromatin remodeling factors. The genomic locus (TgSRCAP) is present as a single copy and contains 16 introns. The predicted cDNA contains an open reading frame of 8,775 bp and encodes a protein of 2,924 amino acids. We have identified additional SRCAP-like sequences in Apicomplexa for comparison by screening genomic databases. An analysis of SRCAP homologues between species reveals signature features that may be indicative of SRCAP members. Expression of mRNA encoding TgSRCAP is upregulated when tachyzoite (invasive form) parasites are induced to differentiate into bradyzoites (encysted form) in vitro. Recombinant TgSRCAP protein is functionally equivalent to the human homologue, being capable of increasing transcription mediated by CREB. |
| Databáze: | OpenAIRE |
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