Ectopic activation of the rice NLR heteropair RGA4/RGA5 confers resistance to bacterial blight and bacterial leaf streak diseases
Autor: | Stella Cesari, Corinne Michel, Véronique Chalvon, Jens Boch, Boris Szurek, Mathilde Hutin, Tuan Tu Tran, Thomas Kroj, Ralf Koebnik |
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Přispěvatelé: | UMR - Interactions Plantes Microorganismes Environnement (UMR IPME), Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Martin-Luther-University Halle-Wittenberg, GRiSP (MENER-GEP New Frontier program), Agropolis Foundation : 0802-023, Genoplante programme (Project 'Interaction Rice Magnaporthe'), Ministere de l'Enseignement superieur et de la Recherche (MESR), INRA CJS grant, Erasmus Mundus Action 2 PANACEA program of the European Community, Vietnam International Education Development (VIED), European Project: 248326,EC:FP7:ICT,FP7-ICT-2009-4,MUNDUS(2010), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine résistance aux maladies [SDV.SA]Life Sciences [q-bio]/Agricultural sciences Xanthomonas Phytopathology and phytopharmacy Transgene Plant Science Biology 01 natural sciences 03 medical and health sciences Transactivation Xanthomonas oryzae TAL effector Gene Expression Regulation Plant Genetics Promoter Regions Genetic Gene pathologie végétale Bacterial leaf streak Disease Resistance Plant Diseases Plant Proteins 2. Zero hunger résistance aux bacteries Effector food and beverages Oryza Cell Biology biology.organism_classification Plants Genetically Modified Genetically modified rice Phytopathologie et phytopharmacie maladie des plantes Agricultural sciences [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy Magnaporthe 030104 developmental biology Sciences agricoles 010606 plant biology & botany |
Zdroj: | Plant Journal Plant Journal, Wiley, 2016, 88 (1), pp.43-55. ⟨10.1111/tpj.13231⟩ Plant Journal, 2016, 88 (1), pp.43-55. ⟨10.1111/tpj.13231⟩ Plant Journal 1 (88), 43-55. (2016) |
ISSN: | 1365-313X 0960-7412 |
DOI: | 10.1111/tpj.13231⟩ |
Popis: | BGPI : Equipe 4 : Interactions Céréales Agents Pathogènes (ICAP); International audience; Bacterial Blight (BB) and Bacterial Leaf Streak (BLS) are important rice diseases caused,respectively, by Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola(Xoc). In both bacteria, Transcription Activator-Like (TAL) effectors are major virulencedeterminants that act by transactivating host genes downstream of Effector-Binding Elements(EBEs) bound in a sequence specific manner. Resistance to Xoo is mostly related to TALeffectors action, either by polymorphisms that prevent induction of susceptibility (S) genes orby executor (R) genes with EBEs embedded in their promoter and that induce cell death andresistance. For Xoc, no resistance sources are known in rice. Here, we investigated whether therecognition of effectors by nucleotide-binding and leucine rich repeat domain immunereceptors (NLRs), the most widespread resistance mechanism in plants, is also able to stop BBand BLS. In one instance, transgenic rice lines harboring the AVR1-CO39 effector gene from therice blast fungus Magnaporthe oryzae, under the control of an inducible promoter, werechallenged with transgenic Xoo and Xoc strains carrying a TAL effector designed to transactivatethe inducible promoter. This induced AVR1-CO39 expression and triggered BB and BLSresistance when the corresponding Pi-CO39 resistance locus was present. In a second example,transactivation of an auto-active NLR by Xoo-delivered designer TAL effectors resulted in BBresistance, demonstrating that NLR-triggered immune responses efficiently control Xoo. Thisforms the foundation for future BB and BLS disease control strategies whereupon endogenousTAL effectors will target synthetic promoter regions of Avr or NLR executor genes. |
Databáze: | OpenAIRE |
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