Quantitative Characterization of Three Carbonic Anhydrase Inhibitors by LESA Mass Spectrometry

Autor:	Eva Illes-Toth, Christopher J. Stubbs, Emma K. Sisley, Jeddidiah Bellamy-Carter, Anna L. Simmonds, Todd H. Mize, Iain B. Styles, Richard J. A. Goodwin, Helen J. Cooper
Rok vydání:	2022
Předmět:	Structural Biology Liquid-Liquid Extraction Solvents Animals Proteins Cattle Carbonic Anhydrase Inhibitors Ligands Spectroscopy Mass Spectrometry
Zdroj:	Journal of the American Society for Mass Spectrometry. 33(7)
ISSN:	1879-1123
Popis:	Liquid extraction surface analysis (LESA) coupled to native mass spectrometry (MS) presents unique analytical opportunities due to its sensitivity, speed, and automation. Here, we examine whether this tool can be used to quantitatively probe protein-ligand interactions through calculation of equilibrium dissociation constants (
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c040622aab221eb88b678d716d50c30f https://pubmed.ncbi.nlm.nih.gov/35675480 Zobrazit plný text záznamu