Peripheral tolerance in T cell receptor-transgenic mice: Evidence for T cell anergy
| Autor: | Dean Falb, Thomas Kamradt, Thomas J. Briner, Cheryl R. Bourque, Mohammad Luqman, Geoffrey H. Sunshine, Malcolm L. Gefter |
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| Rok vydání: | 1996 |
| Předmět: |
Injections
Subcutaneous Receptors Antigen T-Cell alpha-beta T-Lymphocytes T cell Molecular Sequence Data Immunology Cytochrome c Group Mice Transgenic Moths Biology Lymphocyte Activation Epitopes Mice Interleukin 21 Antigen Immune Tolerance medicine Animals Immunology and Allergy Cytotoxic T cell Amino Acid Sequence IL-2 receptor Antigen-presenting cell Clonal Anergy T-cell receptor Peripheral tolerance Thymectomy Molecular biology medicine.anatomical_structure Injections Intravenous |
| Zdroj: | European Journal of Immunology. 26:130-135 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830260120 |
| Popis: | T cell tolerance can be induced in adult mice by injection of soluble antigenic peptide. The underlying mechanism has been difficult to establish in normal mice due to the low precursor frequency of T cells specific for any given antigen. Therefore, we examined peripheral tolerance in mice transgenic for a T cell receptor specific for a cytochrome c peptide bound to I-Ek. Antigen-specific hyporesponsiveness could be induced in the transgenic mice. We followed the transgene-bearing T cells with a clonotypic monoclonal antibody and found similar numbers of clonotypic T cells in tolerized and control mice. To prevent de novo differentiation of T cells we analyzed thymectomized mice in which antigen-specific hyporesponsiveness was induced. Our analysis of thymectomized transgenic mice showed that antigen-specific T cell hyporesponsiveness following injection of peptide intravenously is not caused by gross elimination of T cells. These data provide evidence for the role of anergy in peripheral tolerance. |
| Databáze: | OpenAIRE |
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