Interleukin-8 is a major component of pleural liquid chemotactic activity in a rabbit model of endotoxin pleurisy
| Autor: | M. Sadick, A. M. Boylan, J. M. Hoeffel, Wai Lee Wong, Anan Chuntharapai, K. T. Hartiala, V. C. Broaddus, Caroline Hebert |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Physiology medicine.drug_class Blotting Western Molecular Sequence Data Monoclonal antibody Pleural disease Antigen Desensitization (telecommunications) In vivo Physiology (medical) medicine Animals Interleukin 8 Pleurisy Lagomorpha Base Sequence Chemotactic Factors biology business.industry Interleukin-8 Cell Biology medicine.disease biology.organism_classification Molecular biology Recombinant Proteins Body Fluids Endotoxins Molecular Probes Immunology Pleura Rabbits business |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 267:L137-L144 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.1994.267.2.l137 |
| Popis: | Gram-negative endotoxin induces production of the potent chemotactic factor interleukin-8 (IL-8) in vitro; however, the importance of IL-8 in endotoxin-induced inflammation in vivo is unknown. We asked whether IL-8 is an important contributor to chemotactic activity in acute inflammatory liquids formed in response to endotoxin, and, if present, what concentrations of IL-8 antigen are generated. For these studies, we cloned and expressed rabbit recombinant IL-8 (rrIL-8), developed specific anti-rabbit IL-8 monoclonal antibodies (mAb), and then used these reagents to develop assays to detect rabbit IL-8 bioactivity and measure rabbit IL-8 antigen. Escherichia coli endotoxin (20 ng/ml, n = 4, or 2,000 ng/ml, n = 4) was instilled into the pleural space of eight rabbits for 6 h. Rabbit IL-8 bioactivity in the endotoxin pleurisy samples was assayed by measuring the migration of rabbit neutrophils toward the pleural liquid under two different conditions: 1) after addition of an anti-IL-8 neutralizing mAb and 2) after desensitization of the neutrophils to rrIL-8. Addition of the anti-IL-8 mAb decreased neutrophil migration toward the pleural liquid by 65 +/- 13 and 75 +/- 22% (mean +/- SE, after 20 and 2,000 ng/ml endotoxin, respectively; P < 0.01 compared with a control mAb). Desensitization of neutrophils to rrIL-8 decreased their migration toward the pleural liquid by 72 +/- 5% (P = 0.03, compared with exposure of neutrophils to buffer alone.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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