Construction and characterization of immunoliposomes targeting fibroblast growth factor receptor 3
Autor: | Haotian Ji, Chengjun Yang, Zhong Zheng, Wenbo Zong, Xinxin Wang, Zhuo Zhao, Xi Yang, Yechen Xiao, Qiuju Ran |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
musculoskeletal diseases
lcsh:Biotechnology lcsh:QR1-502 Biophysics Drug resistance Applied Microbiology and Biotechnology lcsh:Microbiology law.invention 03 medical and health sciences 0302 clinical medicine law lcsh:TP248.13-248.65 Single-chain variable fragment 030304 developmental biology 0303 health sciences Liposome Immunoliposomes Chemistry Fibroblast growth factor receptor 3 Fusion protein Cell biology Single chain variable fragment 030220 oncology & carcinogenesis Cancer cell Liposomes Recombinant DNA Original Article Cysteine |
Zdroj: | AMB Express AMB Express, Vol 9, Iss 1, Pp 1-9 (2019) |
ISSN: | 2191-0855 |
Popis: | Fibroblast growth factor receptor 3 (FGFR3) plays an important regulatory role in tumor cell proliferation and drug resistance. FGFR3 is often constitutively active in many tumors. To deliver drugs into tumor cells by targeting FGFR3 will be a promising and potential strategy for cancer therapy. In this study, a novel fusion protein, ScFv-Cys containing a single chain variable fragment (ScFv) and an additional C-terminal cysteine residue, was generated at a rate of 10 mg/L of bacterial culture and purified at 95% by Ni-NTA chromatography. Subsequently, the recombinant ScFv-Cys was coupled with malPEG2000-DSPE and incorporated into liposomes to generate the immunoliposomes. The results indicated that immunoliposomes can specifically deliver the fluorescent molecules, Dio into bladder cancer cells highly expressing FGFR3. In conclusion, we successfully generated FGFR3-specific immunoliposomes, and proved its targeting effect and delivering ability. |
Databáze: | OpenAIRE |
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