Chromatographic Characterization and Process Performance of Column-Packed Anion Exchange Fibrous Adsorbents for High Throughput and High Capacity Bioseparations
| Autor: | Marcelo Fernández-Lahore, Mirna Lorena Sánchez, Poondi Rajesh Gavara, Noor Shad Bibi, Mariano Grasselli |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Materials science
COLUMN PACKING Bioengineering INGENIERÍAS Y TECNOLOGÍAS 02 engineering and technology lcsh:Chemical technology 01 natural sciences Biotecnología Industrial dynamic binding capacity lcsh:Chemistry chemistry.chemical_compound Adsorption Acetone Chemical Engineering (miscellaneous) lcsh:TP1-1185 Fiber Bovine serum albumin Porosity fibrous adsorbent Chromatography biology Ion exchange DYNAMIC BINDING CAPACITY permeability coefficient Process Chemistry and Technology purl.org/becyt/ford/2.9 [https] 010401 analytical chemistry dBc 021001 nanoscience & nanotechnology 0104 chemical sciences FIBROUS ADSORBENT purl.org/becyt/ford/2 [https] lcsh:QD1-999 chemistry biology.protein Theoretical plate 0210 nano-technology PERMEABILITY COEFFICIENT column packing |
| Zdroj: | Processes, Vol 3, Iss 1, Pp 204-221 (2015) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Processes Volume 3 Issue 1 Pages 204-221 |
| ISSN: | 2227-9717 |
| Popis: | Fibrous materials are prominent among novel chromatographic supports for the separation and purification of biomolecules. In this work, strong anion exchange, quaternary ammonium (Q) functional fibrous adsorbents were evaluated with regards to their physical and functional characteristics. A column packed with Q fibrous adsorbent illustrated the good column packing efficiency of theoretical plate height (H) values and higher permeability coefficients (> 0.9 × 10−7 cm2) than commercial adsorbents. For pulse experiments with acetone and lactoferrin as tracers under nonbinding conditions, the total porosity (for acetone) and the interstitial porosity (for lactoferrin) measured 0.97 and 0.47, respectively. The total ionic capacity of the chemically-functionalized Q fiber was 0.51 mmol/mL. The results indicated that the Q fiber had a static binding capacity of 140 mg/mL and a dynamic binding capacity (DBC) of 76 mg/mL for bovine serum albumin (BSA) and showed a linearly-scalable factor (~110 mL) for a column volume with high capacity and high throughput. Furthermore, this adsorptive material had the ability to bind the high molecular weight protein, thyroglobulin, with a capacity of 6 mg/mL. This work demonstrated the column-packed Q fibrous adsorption system as a potential chromatography support that exhibits high capacity at higher flow rates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|