Application of novel vectors for GFP-tagging of proteins to study microtubule-associated proteins
| Autor: | Thierry Doll, Ruedi Meili, Andrew Matus, Stefanie Kaech, Beat Ludin |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary Scyphozoa Microtubule-associated protein Genetic Vectors Green Fluorescent Proteins Molecular Sequence Data Biology Transfection Green fluorescent protein Bimolecular fluorescence complementation Microtubule Genetics Fluorescence microscope Animals Humans Cloning Molecular Cytoskeleton Luminescent Proteins Expression vector Base Sequence fungi General Medicine Molecular biology Cell biology Microscopy Fluorescence Microtubule-Associated Proteins HeLa Cells Fluorescent tag |
| Zdroj: | Gene. 173:107-111 |
| ISSN: | 0378-1119 |
| Popis: | We describe the construction of pBact-NGFP and pBact-CGFP, two expression vectors that incorporate green fluorescent protein (GFP) as a fluorescent tag at the N- or C terminus of the produced protein. When transfected into recipient cells, GFP-tagged proteins can be visualised in the living cells using standard fluorescence microscopy techniques. Using these expression vectors, we have produced GFP-tagged versions of the neuronal microtubule-associated proteins (MAP), MAP2c and Tau34, in a number of different cell types. Both GFP-MAP2c and GFP-Tau34 were fluorescent and retained their ability to bind to microtubules. The pBact-NGFP and pBact-CGFP expression vectors represent a fast and convenient way to produce fluorescently tagged polypeptides of selected sequences encoding whole proteins or fragments for the analysis of function and dynamic events in living cells. |
| Databáze: | OpenAIRE |
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