Foxo1 deletion promotes the growth of new lymphatic valves
| Autor: | Joshua P. Scallan, Jorge A. Castorena-Gonzalez, Michael J. Davis, Luz A. Knauer, Huayan Hou, Ying Yang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Mice
Knockout Gene knockdown biology Forkhead Box Protein O1 government.form_of_government Forkhead Transcription Factors FOXO1 General Medicine Congenital lymphedema Andrology Mice Lymphatic Endothelium Lymphatic system Downregulation and upregulation biology.protein government Animals Lymphangiogenesis FOXC2 Transcription factor Gene Deletion Lymphatic Vessels Research Article |
| Zdroj: | J Clin Invest |
| ISSN: | 1558-8238 |
| DOI: | 10.1172/jci142341 |
| Popis: | Patients with congenital lymphedema suffer from tissue swelling in part due to mutations in genes regulating lymphatic valve development. Lymphatic valve leaflets grow and are maintained throughout life in response to oscillatory shear stress (OSS), which regulates gene transcription in lymphatic endothelial cells (LECs). Here, we identified the first transcription factor, Foxo1, that repressed lymphatic valve formation by inhibiting the expression of valve-forming genes. We showed that both embryonic and postnatal ablation of Foxo1 in LECs induced additional valve formation in postnatal and adult mice in multiple tissues. Our quantitative analyses revealed that after deletion, the total number of valves in the mesentery was significantly (P < 0.01) increased in the Foxo1(LEC-KO) mice compared with Foxo1(fl/fl) controls. In addition, our quantitative real-time PCR (RT-PCR) data from cultured LECs showed that many valve-forming genes were significantly (P < 0.01) upregulated upon knockdown of FOXO1. To confirm our findings in vivo, rescue experiments showed that Foxc2(+/–) mice, a model of lymphedema-distichiasis, had 50% fewer lymphatic valves and that the remaining valves exhibited backleak. Both valve number and function were completely restored to control levels upon Foxo1 deletion. These findings established FOXO1 as a clinically relevant target to stimulate de novo lymphatic valve formation and rescue defective valves in congenital lymphedema. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|