Intestinal Ischemic Preconditioning After Ischemia/Reperfusion Injury in Rat Intestine: Profiling Global Gene Expression Patterns
| Autor: | Rosemary A. Kozar, Steven T. Lott, Bruce C. Kone, Ravi S. Radhakrishnan, Stacey D. Moore-Olufemi, Shodimu Emmanuel Olufemi, Shinil K. Shah, Frederick A. Moore, Norio Sato, Fernando Jimenez, Charles S. Cox |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Pathology medicine.medical_specialty Necrosis Physiology Ischemia Rats Sprague-Dawley Random Allocation Intestinal mucosa Ileum Reference Values parasitic diseases Gene expression medicine Animals Cluster Analysis cardiovascular diseases Intestinal Mucosa Ischemic Preconditioning Oligonucleotide Array Sequence Analysis Probability Regulation of gene expression Analysis of Variance Reverse Transcriptase Polymerase Chain Reaction business.industry Gene Expression Profiling Gastroenterology Microarray Analysis medicine.disease Rats Gene expression profiling Disease Models Animal Gene Expression Regulation Reperfusion Injury RNA Ischemic preconditioning medicine.symptom business Reperfusion injury |
| Zdroj: | Digestive Diseases and Sciences. 55:1866-1877 |
| ISSN: | 1573-2568 0163-2116 |
| DOI: | 10.1007/s10620-009-0980-4 |
| Popis: | Intestinal ischemia/reperfusion (IR) injury involves activation of inflammatory mediators, mucosal necrosis, ileus, and alteration in a variety of gene products. Ischemic preconditioning (IPC) reduced all the effects of intestinal injury seen in IR. In an effort to investigate the molecular mechanisms responsible for the protective effects afforded by IPC, we sought to characterize the global gene expression pattern in rats subjected to IPC in the setting of IR injury.Rats were randomized into five groups: (1) Sham, (2) IPC only (3) IR, (4) Early IPC + IR (IPC --IR), and (5) Late IPC + IR (IPC --24 h --IR). At 6 h after reperfusion, ileum was harvested for total RNA isolation, pooled, and analyzed on complementary DNA (cDNA) microarrays with validation using real-time polymerase chain reaction (PCR). Significance Analysis of Microarray (SAM) software was used to determine statistically significant changes in gene expression.Early IPC + IR had 5,167 induced and 4 repressed genes compared with the other groups. SAM analysis revealed 474 out of 10,000 genes differentially expressed among the groups. Early and Late IPC + IR had more genes involved in redox hemostasis, the immune/inflammatory response, and apoptosis than either the IPC only or IR alone groups.The transcriptional profile suggests that IPC exerts its protective effects by regulating the gene response to injury in the intestine. |
| Databáze: | OpenAIRE |
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