Up-regulation of alpha1-microglobulin by hemoglobin and reactive oxygen species in hepatoma and blood cell lines
| Autor: | Tor Olofsson, Bo Åkerström, Maria Allhorn, Magnus G. Olsson |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Carcinoma
Hepatocellular Erythrocytes up-regulated expression Alpha (ethology) Heme Biochemistry Methemoglobin Cell Line chemistry.chemical_compound Hemoglobins Physiology (medical) Alpha-Globulins Humans RNA Messenger Alpha globulin chemistry.chemical_classification Reactive oxygen species biology Biochemistry and Molecular Biology ROS U937 Cells hemoglobin Molecular biology Up-Regulation Oxygen chemistry Gene Expression Regulation Catalase alpha(1)-microglobulin biology.protein Hemoglobin blood cells hepatoma cells Alpha-1-microglobulin K562 Cells Reactive Oxygen Species Oxidation-Reduction |
| Zdroj: | Free Radical Biology & Medicine; 42(6), pp 842-851 (2007) |
| ISSN: | 0891-5849 |
| Popis: | alpha(1)-Microglobulin is a 26-kDa glycoprotein synthesized in the liver, secreted to the blood, and rapidly distributed to the extravascular compartment of all tissues. Recent results show that alpha(1)-microglobulin has heme-binding and heme-degrading properties and it has been suggested that the protein is involved in the defense against oxidation by heme and reactive oxygen species. In the present study the influence of hemoglobin and reactive oxygen species (ROS) on the cellular expression of alpha(1)-microglobulin was investigated. Oxy- and methemoglobin, free heme, and Fenton reaction-induced hydroxyl radicals induced a dose-dependent up-regulation of alpha(1)-microglobutin on both mRNA and protein levels in hepatoma cells and an increased secretion of alpha(1)-microglobulin. The up-regulation was reversed by the addition of catalase and ascorbate, and by reacting hemoglobin with cyanide which prevents redox reactions. Furthermore, the blood cell lines U937 and K562 expressed alpha(1)-microglobulin at low levels, and this expression increased up to 11-fold by the addition of hemoglobin. These results suggest that a-l-microglobulin expression is induced by ROS, arising from redox reactions of hemoglobin or from other sources and are consistent with the hypothesis that alpha(1)-microglobulin participates in the defense against oxidation by hemoglobin, heme, and reactive oxygen species. (c) 2007 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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