A diatom gene regulating nitric-oxide signaling and susceptibility to diatom-derived aldehydes
| Autor: | Chris Bowler, Assaf Vardi, Paul G. Falkowski, Donald J. Hirsh, Clifford J. Kwityn, Kay D. Bidle, Stephanie E.M. Thompson, James A. Callow |
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| Rok vydání: | 2008 |
| Předmět: |
0106 biological sciences
EVO_ECOL Chloroplasts Transgene Ribosome biogenesis Context (language use) Nitric Oxide 01 natural sciences General Biochemistry Genetics and Molecular Biology Superoxide dismutase 03 medical and health sciences GTP-Binding Proteins Botany Cell Adhesion 14. Life underwater Phaeodactylum tricornutum Plastid Photosynthesis Gene 030304 developmental biology Diatoms 0303 health sciences Aldehydes biology Agricultural and Biological Sciences(all) Biochemistry Genetics and Molecular Biology(all) fungi biology.organism_classification Cell biology Diatom Gene Expression Regulation SIGNALING biology.protein General Agricultural and Biological Sciences Heat-Shock Response 010606 plant biology & botany Signal Transduction |
| Zdroj: | Current biology : CB. 18(12) |
| ISSN: | 0960-9822 |
| Popis: | Summary Diatoms are unicellular phytoplankton accounting for ∼40% of global marine primary productivity [1], yet the molecular mechanisms underlying their ecological success are largely unexplored. We use a functional-genomics approach in the marine diatom Phaeodactylum tricornutum to characterize a novel protein belonging to the widely conserved YqeH subfamily [2] of GTP-binding proteins thought to play a role in ribosome biogenesis [3], sporulation [4], and nitric oxide (NO) generation [5]. Transgenic diatoms overexpressing this gene, designated PtNOA , displayed higher NO production, reduced growth, impaired photosynthetic efficiency, and a reduced ability to adhere to surfaces. A fused YFP-PtNOA protein was plastid localized, distinguishing it from a mitochondria-localized plant ortholog. PtNOA was upregulated in response to the diatom-derived unsaturated aldehyde 2E,4E/Z-decadienal (DD), a molecule previously shown to regulate intercellular signaling, stress surveillance [6], and defense against grazers [7]. Overexpressing cell lines were hypersensitive to sublethal levels of this aldehyde, manifested by altered expression of superoxide dismutase and metacaspases, key components of stress and death pathways [8, 9]. NOA-like sequences were found in diverse oceanic regions, suggesting that a novel NO-based system operates in diatoms and may be widespread in phytoplankton, providing a biological context for NO in the upper ocean [10]. |
| Databáze: | OpenAIRE |
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